|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Rabbit|
|Western Blotting (WB)||284 Antibodies||123 Antibodies||103 Antibodies||22 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies||14 Antibodies||22 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||56 Antibodies||24 Antibodies||23 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||115 Antibodies||60 Antibodies||21 Antibodies||67 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling Images
|Specificity||This antibody is directed against the p44 MAP Kinase (ERK1) protein.|
Species reactivity (expected):Bovine, Rabbit, Mouse, Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein|
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|Alternative Name||MAPK3 / ERK1 (MAPK3 Antibody Abstract)|
|Background||Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
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ELISA: 1/2000 - 1/10000. Western Blot: 1/1000 - 1/2000. Immunoprecipitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.1 M Tris HCl, 0.15 M Sodium Chloride, pH 7.5 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
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