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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||29 Antibodies||41 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||40 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||28 Antibodies|
|Immunofluorescence (IF)||43 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies|
|Immunohistochemistry (IHC)||70 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies|
|Immunoprecipitation (IP)||22 Antibodies|
|Western Blotting (WB)||112 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK3 antibody is conjugated to Biotin Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling Images
|Specificity||This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.|
|Characteristics||Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK3 / ERK1 (MAPK3 Antibody Abstract)|
|Background||Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
|Buffer||PBS/0.09 % Na-Azide/PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-80 °C|
Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|