You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Dog (Canine) MAPK3 antibody for Enzyme Immunoassay

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
pThr-Glu-pTyr
55
44
42
39
24
20
15
14
13
9
9
8
8
5
5
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
389
173
149
25
23
22
19
19
11
9
6
5
4
3
3
2
2
2
2
2
1
1
Host
Mouse
308
94
21
2
1
Clonality
Monoclonal
Conjugate
This MAPK3 antibody is conjugated to Biotin
21
20
19
13
12
12
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
322
167
145
75
57
48
39
33
29
18
9
5
4
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN126829
$ 610.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4

Similar anti-MAPK3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 25 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 22 Antibodies 22 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 103 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 4 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope pThr-Glu-pTyr
(55), (44), (42), (39), (24), (20), (15), (14), (13), (9), (9), (8), (8), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(389), (173), (149), (25), (23), (22), (19), (19), (11), (9), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(308), (94), (21), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK3 antibody is conjugated to Biotin
(21), (20), (19), (13), (12), (12), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(322), (167), (145), (75), (57), (48), (39), (33), (29), (18), (9), (5), (4), (3), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2.
Characteristics Synonyms: ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK, Extracellular signal regulated kinase 1/2,Insulin stimulated MAP2 kinase, Microtubule associated protein 2 kinase, Mitogenactivated protein kinase 1/2/3
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjugated to KLH
Clone 12D4
Isotype IgG1

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from pervanadate-treated HepG2 cells. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 10^6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS/0.09 % Na-Azide/PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-80 °C
Storage Comment Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months