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anti-Dog (Canine) MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
pThr202, Tyr204
58
44
42
38
27
20
20
15
14
13
9
8
8
8
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
454
216
155
26
22
21
19
19
16
12
7
6
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
317
151
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
384
184
151
95
67
57
50
33
30
18
10
5
4
3
2
2
2
1
1
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Catalog No. ABIN372623
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088411616 ABIN126829 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.00088411616 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4

Top referenced anti-MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-MAPK3 Antibodies

Application / Reactivity Dog (Canine) Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Xenopus laevis Non Human Primates Zebrafish (Danio rerio)
Western Blotting (WB) 8 Antibodies 351 Antibodies 164 Antibodies 106 Antibodies 23 Antibodies 22 Antibodies 22 Antibodies 1 Antibodies 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 5 Antibodies 38 Antibodies 14 Antibodies 22 Antibodies 1 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies 29 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope pThr202, Tyr204
(58), (44), (42), (38), (27), (20), (20), (15), (14), (13), (9), (8), (8), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(454), (216), (155), (26), (22), (21), (19), (19), (16), (12), (7), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(317), (151), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(384), (184), (151), (95), (67), (57), (50), (33), (30), (18), (10), (5), (4), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN372623) Images
Specificity Recognizes the ~42k-44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and ZebrafishSpecies reactivity (tested):Human and Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN372623) Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002, Ahn, 1993, Tanoue and Nishida, 2003, Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
NCBI Accession NP_002737
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN372623) Images back to top
Application Notes Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN372623) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-MAPK3 Antibody (ABIN372623)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Tanoue, Nishida: "Molecular recognitions in the MAP kinase cascades." in: Cellular signalling, Vol. 15, Issue 5, pp. 455-62, 2003

Johnson, Lapadat: "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases." in: Science (New York, N.Y.), Vol. 298, Issue 5600, pp. 1911-2, 2002

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002

Ahn: "The MAP kinase cascade. Discovery of a new signal transduction pathway." in: Molecular and cellular biochemistry, Vol. 127-128, pp. 201-9, 1994

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN372623) back to top
Supplier Images
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (pThr202) (ABIN372623) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (Tyr204), (pThr202) antibody