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anti-Fruit Fly (Drosophila melanogaster) MAPK3 antibody for Immunohistochemistry

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Reactivity
Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
389
173
149
24
22
21
18
18
11
10
6
5
4
3
3
2
2
2
2
2
1
1
Host
Rabbit
307
95
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
21
21
19
13
12
12
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
322
167
144
75
57
49
38
33
28
18
10
6
4
3
2
1
1
1
Supplier
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Catalog No. ABIN361833
$ 336.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011453824 ABIN2486604 ICC FACS IF IHC WB Atto 390 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486606 ICC FACS IF IHC WB Atto 565 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486607 ICC FACS IF IHC WB Atto 594 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486605 ICC FACS IF IHC WB Atto 488 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486609 ICC FACS IF IHC WB Atto 655 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486608 ICC FACS IF IHC WB Atto 633 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486611 ICC FACS IF IHC WB Atto 700 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486610 ICC FACS IF IHC WB Atto 680 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486918 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486922 ICC FACS IF IHC WB HRP Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486920 ICC FACS IF IHC WB Biotin Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486921 ICC FACS IF IHC WB FITC Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486919 ICC FACS IF IHC WB APC Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486923 ICC FACS IF IHC WB PE-Atto 594 Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486924 ICC FACS IF IHC WB PerCP Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486925 ICC FACS IF IHC WB PE Rabbit Log in to see Polyclonal 3
0.0011453824 ABIN2486926 ICC FACS IF IHC WB Streptavidin Rabbit Log in to see Polyclonal 3

Top referenced anti-MAPK3 antibody for Immunohistochemistry

Similar anti-MAPK3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Fruit Fly (Drosophila melanogaster) Sheep (Ovine) Xenopus laevis
Western Blotting (WB) 290 Antibodies 123 Antibodies 103 Antibodies 23 Antibodies 22 Antibodies 19 Antibodies 19 Antibodies 22 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 37 Antibodies 14 Antibodies 22 Antibodies 1 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 57 Antibodies 24 Antibodies 23 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Reactivity Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis
(389), (173), (149), (24), (22), (21), (18), (18), (11), (10), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(307), (95), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(21), (21), (19), (13), (12), (12), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(322), (167), (144), (75), (57), (49), (38), (33), (28), (18), (10), (6), (4), (3), (2), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN361833) Images
Specificity Detects ~44kda (ERK1) and ~42 kDa (ERK2).
Purification Rabbit Antiserum
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN361833) Images back to top
Antigen
Alternative Name ERK1 (MAPK3 Antibody Abstract)
Background The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Cellular Localization: Cytoplasm | Nucleus
Gene ID 50689
NCBI Accession NP_059043
UniProt P21708
Research Area Stem Cells, Alzheimer's Disease, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN361833) Images back to top
Application Notes Recommended Dilution: WB (1:5000), IHC (1:100), ICC/IF (1:100), FCM (1:100), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN361833) Images back to top
Format Liquid
Buffer Rabbit Antiserum
Storage -20 °C

References for anti-MAPK3 Antibody (ABIN361833)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Product cited in:

Revuelta-López, Cal, Herraiz-Martínez, de Gonzalo-Calvo, Nasarre, Roura, Gálvez-Montón, Bayes-Genis, Badimon, Hove-Madsen, Llorente-Cortés: "Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes." in: Journal of molecular and cellular cardiology, Vol. 85, pp. 25-36, 2015

Thon, Hosoi, Yoshii, Ozawa: "Leptin induced GRP78 expression through the PI3K-mTOR pathway in neuronal cells." in: Scientific reports, Vol. 4, pp. 7096, 2014

Bhat, Gomis, Potter, Tikoo: "Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages." in: Molecular immunology, Vol. 47, Issue 6, pp. 1337-46, 2010

Enikanolaiye, Larivière, Troy, Arabzadeh, Atasoy, Turksen: "Involucrin-claudin-6 tail deletion mutant (CDelta206) transgenic mice: a model of delayed epidermal permeability barrier formation and repair." in: Disease models & mechanisms, Vol. 3, Issue 3-4, pp. 167-80, 2010

Background publications

Chuderland, Marmor, Shainskaya, Seger: "Calcium-mediated interactions regulate the subcellular localization of extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 283, Issue 17, pp. 11176-88, 2008

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Zhuang, Schnellmann: "A death-promoting role for extracellular signal-regulated kinase." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 3, pp. 991-7, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN361833) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (ABIN361833) Erk1 2 Western Blotting 1 in 1000 human cell line mix 10ug.