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anti-Human MAPK3 antibody for Enzyme Immunoassay

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
pTyr204
58
43
42
39
27
20
20
15
14
13
9
9
8
8
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
454
217
156
27
23
22
19
19
16
13
7
6
4
4
4
4
3
3
2
2
2
1
1
Host
Rabbit
317
151
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
385
184
151
96
67
57
50
33
30
18
9
6
4
3
2
2
2
1
1
Supplier
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Catalog No. ABIN358533
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN359630 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 4
0.0008870071 ABIN782962 EIA WB Goat N-Term Log in to see Polyclonal 1
0.0008870071 ABIN374560 EIA WB Goat N-Term Log in to see Polyclonal 1
0.0008870071 ABIN567619 EIA IF WB Mouse IgG2b Log in to see AT1A2 4
0.0008870071 ABIN126829 EIA IHC (fro) IF IP WB Biotin Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.0008870071 ABIN401356 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008870071 ABIN126831 EIA IHC (fro) IF IP WB Mouse IgG1 pThr-Glu-pTyr Log in to see 12D4
0.0008870071 ABIN782433 EIA IF IHC (p) WB Mouse IgG2a AA 279-379 Log in to see 3C9
0.0008870071 ABIN1108133 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1-00E-005

Top referenced anti-MAPK3 antibody for Enzyme Immunoassay

Similar anti-MAPK3 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
Dot Blot (DB) 18 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 4 Antibodies
ELISA 165 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 31 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 58 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 127 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 38 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope pTyr204
(58), (43), (42), (39), (27), (20), (20), (15), (14), (13), (9), (9), (8), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(454), (217), (156), (27), (23), (22), (19), (19), (16), (13), (7), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(317), (151), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(385), (184), (151), (96), (67), (57), (50), (33), (30), (18), (9), (6), (4), (3), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) Images
Specificity This antibody detects ERK1 pThr202/pTyr204.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated Thr202 and Tyr204 of human ERK1.
Isotype Ig

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Molecular Weight 43136 Da
Gene ID 5595, 9606
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN358533) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN358533) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAPK3 Antibody (ABIN358533)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Background publications

Munshi, Wu, Mukhopadhyay, Ottaviano, Sassano, Koblinski, Platanias, Stack et al.: "Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ..." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39042-50, 2004

Mukherjee, Huang, Petkova, Albanese, Pestell, Braunstein, Christ, Wittner, Lisanti, Berman, Weiss, Tanowitz: "Trypanosoma cruzi infection activates extracellular signal-regulated kinase in cultured endothelial and smooth muscle cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5274-82, 2004

Sebkova, Pellicanò, Monteleone, Grazioli, Guarnieri, Imeneo, Pallone, Luzza: "Extracellular signal-regulated protein kinase mediates interleukin 17 (IL-17)-induced IL-8 secretion in Helicobacter pylori-infected human gastric epithelial cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5019-26, 2004

Huang, Chang, Liu: "Basic fibroblast growth factor antagonizes activin A-mediated growth inhibition and hemoglobin synthesis in K562 cells by activating ERK1/2 and deactivating p38 MAP kinase." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1247-52, 2004

Mizuno, Kadowaki, Demura, Ameshima, Miyamori, Ishizaki: "p42/44 Mitogen-activated protein kinase regulated by p53 and nitric oxide in human pulmonary arterial smooth muscle cells." in: American journal of respiratory cell and molecular biology, Vol. 31, Issue 2, pp. 184-92, 2004

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) back to top
Supplier Images
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (pTyr204) (ABIN358533) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (pTyr204) antibody