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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||4 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||30 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||34 Antibodies|
|Immunofluorescence (IF)||76 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies|
|Immunohistochemistry (IHC)||115 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||56 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||284 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies|
|Conjugate||This MAPK3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAPK3 AntibodyTarget Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) Images
|Specificity||This antibody detects ERK1 pThr202/pTyr204.|
|Purification||Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated Thr202 and Tyr204 of human ERK1.|
Target Details MAPK3Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) Images back to top
|Alternative Name||MAPK3 / ERK1 (MAPK3 Antibody Abstract)|
|Background||MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK|
|Molecular Weight||43136 Da|
|Gene ID||5595, 9606|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN358533) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN358533) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MAPK3 Antibody (ABIN358533)Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Mukherjee, Huang, Petkova et al.: "Trypanosoma cruzi infection activates extracellular signal-regulated kinase in cultured endothelial and smooth muscle cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5274-82, 2004 (PubMed).
Sebkova, Pellicanò, Monteleone et al.: "Extracellular signal-regulated protein kinase mediates interleukin 17 (IL-17)-induced IL-8 secretion in Helicobacter pylori-infected human gastric epithelial cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5019-26, 2004 (PubMed).
Huang, Chang, Liu: "Basic fibroblast growth factor antagonizes activin A-mediated growth inhibition and hemoglobin synthesis in K562 cells by activating ERK1/2 and deactivating p38 MAP kinase." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1247-52, 2004 (PubMed).
Munshi, Wu, Mukhopadhyay et al.: "Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ..." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39042-50, 2004 (PubMed).
Mizuno, Kadowaki, Demura et al.: "p42/44 Mitogen-activated protein kinase regulated by p53 and nitric oxide in human pulmonary arterial smooth muscle cells." in: American journal of respiratory cell and molecular biology, Vol. 31, Issue 2, pp. 184-92, 2004 (PubMed).
ImagesProduct Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN358533) back to top