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anti-Human MAPK3 antibody for Immunofluorescence

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
AA 325-345
58
44
42
39
27
20
20
15
14
13
9
9
8
8
5
5
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
454
216
155
27
22
22
19
19
16
12
7
6
4
4
4
4
3
2
2
2
2
1
1
Host
Mouse
318
150
21
2
1
Clonality
Monoclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
384
184
150
95
67
57
49
33
30
18
10
6
4
3
2
2
2
1
1
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Catalog No. ABIN967952
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32215732 ABIN967700 IF IP IHC WB Mouse IgG1 AA 325-345 Log in to see MK12 5
0.32215732 ABIN967701 IF IP IHC WB Mouse IgG1 AA 325-345 Log in to see MK12 5
0.32215732 ABIN567619 EIA IF WB Mouse IgG2b Log in to see AT1A2 4
0.32215732 ABIN782433 EIA IF IHC (p) WB Mouse IgG2a AA 279-379 Log in to see 3C9
0.32215732 ABIN2706122 IC IF WB Rabbit Center Log in to see Polyclonal
0.32215732 ABIN1870387 IF IHC WB Rabbit IgG pTyr204 Log in to see Polyclonal
0.32215732 ABIN1870385 IF IHC WB Rabbit IgG pThr202 Log in to see Polyclonal
0.32215732 ABIN934066 IF ELISA WB Mouse IgG2b kappa AA 1-137 Log in to see AT1A2
0.32215732 ABIN519216 IF ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 1F7
0.32215732 ABIN519214 IF ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 1B1
0.32215732 ABIN1108133 EIA IF IHC (p) WB Mouse IgG1 Log in to see 1-00E-005
0.32215732 ABIN519217 IF ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 3D6
0.32215732 ABIN519215 IF ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 1E2
0.32215732 ABIN562406 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 279-379, partial Log in to see 3C9
0.32215732 ABIN2425003 IF IHC WB Rabbit IgG pTyr204 Log in to see Polyclonal
0.32215732 ABIN2425001 IF IHC WB Rabbit IgG pThr202 Log in to see Polyclonal
0.32215732 ABIN3019887 IF IHC WB Rabbit pThr202 Log in to see Polyclonal
0.32215732 ABIN3019890 IF IHC WB Rabbit pTyr204 Log in to see Polyclonal
0.001082034 ABIN264862 IHC (fro) IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 6
0.001082034 ABIN393379 IF WB Mouse IgG2a kappa AA 279-379 Log in to see 1E2 5

Top referenced anti-MAPK3 antibody for Immunofluorescence

Similar anti-MAPK3 Antibodies

Application / Reactivity Dog (Canine) Human Mouse (Murine) Rat (Rattus) Chicken Frog
Western Blotting (WB) 8 Antibodies 351 Antibodies 164 Antibodies 106 Antibodies 23 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 5 Antibodies 38 Antibodies 14 Antibodies 22 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies 29 Antibodies 28 Antibodies 1 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 127 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope AA 325-345
(58), (44), (42), (39), (27), (20), (20), (15), (14), (13), (9), (9), (8), (8), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(454), (216), (155), (27), (22), (22), (19), (19), (16), (12), (7), (6), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Host Mouse
(318), (150), (21), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(384), (184), (150), (95), (67), (57), (49), (33), (30), (18), (10), (6), (4), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN967952) Images
Cross-Reactivity Mouse (Murine), Human, Chicken, Dog (Canine), Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ERK1 aa. 325-345
Clone MK1
Isotype IgG1

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling References for anti-MAPK3 Antibody (ABIN967952) Images back to top
Antigen
Alternative Name ERK1 (MAPK3 Antibody Abstract)
Background The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphotases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kD).
Molecular Weight 44 kDa
Research Area Stem Cells, Alzheimer's Disease, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling References for anti-MAPK3 Antibody (ABIN967952) Images back to top
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details References for anti-MAPK3 Antibody (ABIN967952) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-MAPK3 Antibody (ABIN967952)

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling Images back to top
Product cited in:

Radoja, Saio, Schaer, Koneru, Vukmanovic, Frey: "CD8(+) tumor-infiltrating T cells are deficient in perforin-mediated cytolytic activity due to defective microtubule-organizing center mobilization and lytic granule exocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5042-51, 2001

Clark, Hynes: "Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14814-8, 1996

Cobb, Goldsmith: "How MAP kinases are regulated." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 14843-6, 1995

Boulton, Cobb: "Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies." in: Cell regulation, Vol. 2, Issue 5, pp. 357-71, 1991

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling References for anti-MAPK3 Antibody (ABIN967952) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (AA 325-345) (ABIN967952) Western blot analysis of ERK1 on a rat cerebrum lysate. 1:4000 (lane 1), 1:8000 (lane...
Immunofluorescence (IF) image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (AA 325-345) (ABIN967952) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (AA 325-345) (ABIN967952) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 325-345) antibody (Image 3)