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anti-Rabbit MAPK3 antibody for Enzyme Immunoassay

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • Erk-1
  • ERK-1
  • Erk1
  • ERK1
  • ERT2
  • Ert2
  • Esrk1
  • fi06b09
  • HS44KDAP
  • HUMKER1A
  • MAPK1
  • mitogen-activated protein kinase 3
  • MNK1
  • Mnk1
  • Mtap2k
  • p44
  • p44-ERK1
  • p44-MAPK
  • p44erk1
  • P44ERK1
  • p44mapk
  • P44MAPK
  • PRKM3
  • Prkm3
  • T6D9.4
  • Tb08.10J17.940
  • wu:fi06b09
  • zERK1
Epitope
C-Term
54
44
42
39
24
20
15
14
13
9
9
8
8
5
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
389
173
149
24
23
22
19
19
11
10
6
5
4
3
3
2
2
2
2
1
1
1
Host
Rabbit
307
95
21
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
21
21
19
13
12
12
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
322
167
145
76
57
48
39
33
29
18
9
6
4
3
2
1
1
1
Supplier
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Catalog No. ABIN401356
$ 456.50
Plus shipping costs $45.00

Similar anti-MAPK3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Rabbit
Western Blotting (WB) 290 Antibodies 123 Antibodies 103 Antibodies 22 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 37 Antibodies 14 Antibodies 22 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 57 Antibodies 24 Antibodies 23 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 121 Antibodies 60 Antibodies 21 Antibodies 67 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope C-Term
(54), (44), (42), (39), (24), (20), (15), (14), (13), (9), (9), (8), (8), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(389), (173), (149), (24), (23), (22), (19), (19), (11), (10), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(307), (95), (21), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(21), (21), (19), (13), (12), (12), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(322), (167), (145), (76), (57), (48), (39), (33), (29), (18), (9), (6), (4), (3), (2), (1), (1), (1)
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity This antibody is directed against the p44 MAP Kinase (ERK1) protein.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Rabbit, Mouse, Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
NCBI Accession NP_001035145
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes ELISA: 1/2000 - 1/10000. Western Blot: 1/1000 - 1/2000. Immunoprecipitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.1 M Tris HCl, 0.15 M Sodium Chloride, pH 7.5 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

Images

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Handling back to top
Supplier Images
 image for anti-MAPK3 antibody (Mitogen-Activated Protein Kinase 3) (C-Term) (ABIN401356) anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (C-Term) antibody