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anti-Rat (Rattus) MAPK3 antibody for Functional Studies

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • ERK1
  • fi06b09
  • wu:fi06b09
  • zERK1
  • ERK-1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • MAPK1
  • MNK1
  • Tb08.10J17.940
  • mitogen-activated protein kinase 3
  • MAP kinase-like orf; aka CEK1; one of two protein kinase genes similar to S. cerevisiae MAP kinases FUS3 (YBL016W) and KSS1 (YGR040W) involved in pheromone signal transduction
  • mitogen activated protein kinase 3
  • MPK3
  • MAPK3
  • ERK1
  • Mapk3
  • mapk3
  • Tb927.8.3550
  • Tc00.1047053509475.10
Epitope
AA 372-406
48
47
40
37
21
21
20
16
15
14
14
9
8
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Mouse (Murine), Rabbit, Rat (Rattus)
476
269
202
35
26
26
24
22
20
20
13
11
10
9
7
5
3
3
2
2
2
2
1
1
1
1
Host
Rabbit
320
156
22
2
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
23
23
21
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
396
186
164
98
75
56
47
46
39
17
13
9
6
4
3
2
2
2
1
Supplier
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Catalog No. ABIN199418
$ 837.83
Plus shipping costs $45.00

Similar anti-MAPK3 Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus) Rabbit Hamster
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 5 Antibodies
Western Blotting (WB) 207 Antibodies 140 Antibodies 5 Antibodies 4 Antibodies
Immunoprecipitation (IP) 29 Antibodies 29 Antibodies 5 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies 35 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 82 Antibodies 98 Antibodies 2 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope AA 372-406
(48), (47), (40), (37), (21), (21), (20), (16), (15), (14), (14), (9), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Mouse (Murine), Rabbit, Rat (Rattus)
(476), (269), (202), (35), (26), (26), (24), (22), (20), (20), (13), (11), (10), (9), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(320), (156), (22), (2), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(23), (23), (21), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(396), (186), (164), (98), (75), (56), (47), (46), (39), (17), (13), (9), (6), (4), (3), (2), (2), (2), (1)
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity Recognizes rat MAPK1/Erk1 at 44kD and MAPK2/Erk2 at 42kD. Species cross-reactivity: Human, mouse, chicken and starfish. Species sequence Homology: Chinese hamster: 35/35, mouse: 34/35, human: 32/35.
Predicted Reactivity Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%) Monkey, Bovine, Dog, Bat, Horse, Platypus (97%) Human, Gorilla, Gibbon, Elephant (94%) Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%) Xenopus (84%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide (CGG-PFTFDMELDDLPKERLKELIFQETARFQPGAPEAP) corresponding to the C-terminal 35 amino acids of rat 44kD MAP Kinase 2/ Erk2 (KLH coupled). Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Hamster, Panda, Rabbit, Opossum (100%), Monkey, Bovine, Dog, Bat, Horse, Platypus (97%), Human, Gorilla, Gibbon, Elephant (94%), Orangutan, Pig, Turkey, Chicken, Pufferfish, Zebrafish (87%), Xenopus (84%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3
Gene ID 5595
UniProt P27361
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes Approved: Func, IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.1-2 μg/mL detects MAP kinases in RIPA lysates of mouse 3T3/A31 fibroblasts and L6 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MAP Kinase 1/2 (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation Kinase Assay: 4 μg immunoprecipitates active MAP kinases from a lysate of NGF-stimulated (50 ng/mL) PC-12 cells, as demonstrated using the MAPK Immunoprecipitation Kinase Cascade Kit.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 0.1 mM EDTA.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.