anti-Xenopus laevis MAPK3 antibody for Immunohistochemistry (Frozen Sections)

Recommended MAPK3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • ERK1
  • fi06b09
  • wu:fi06b09
  • zERK1
  • ERK-1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • MAPK1
  • MNK1
  • Tb08.10J17.940
  • mitogen-activated protein kinase 3
  • MAP kinase-like orf; aka CEK1; one of two protein kinase genes similar to S. cerevisiae MAP kinases FUS3 (YBL016W) and KSS1 (YGR040W) involved in pheromone signal transduction
  • mitogen activated protein kinase 3
  • MPK3
  • MAPK3
  • ERK1
  • Mapk3
  • mapk3
  • Tb927.8.3550
  • Tc00.1047053509475.10
Epitope
Tyr204, pThr202
48
48
46
39
21
21
19
16
15
14
9
8
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
486
260
194
34
25
23
23
23
20
20
13
12
11
10
9
7
4
4
3
2
2
2
1
1
1
Host
Rabbit
324
160
20
6
1
Clonality
Polyclonal
Conjugate
This MAPK3 antibody is un-conjugated
26
24
22
16
12
12
6
6
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
404
182
163
101
86
57
56
47
39
16
13
8
7
3
3
2
2
2
1
1
Supplier
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Catalog No. ABIN5551547
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551325 IF IHC (fro) IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antibodies
Epitope Tyr204, pThr202
(48), (48), (46), (39), (21), (21), (19), (16), (15), (14), (9), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(486), (260), (194), (34), (25), (23), (23), (23), (20), (20), (13), (12), (11), (10), (9), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(324), (160), (20), (6), (1)
Clonality
Polyclonal
Conjugate This MAPK3 antibody is un-conjugated
(26), (24), (22), (16), (12), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(404), (182), (163), (101), (86), (57), (56), (47), (39), (16), (13), (8), (7), (3), (3), (2), (2), (2), (1), (1)
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Product Details anti-MAPK3 Antibody

Target Details MAPK3 Application Details Handling Images
Specificity Recognizes the ~42k-44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and ZebrafishSpecies reactivity (tested):Human and Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.
Isotype IgG

Target Details MAPK3

Product Details anti-MAPK3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK3 / ERK1 (MAPK3 Antibody Abstract)
Background Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002, Ahn, 1993, Tanoue and Nishida, 2003, Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK
Gene ID 5595
NCBI Accession NP_002737
UniProt P27361
Research Area Inflammation, Signaling
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-MAPK3 Antibody Target Details MAPK3 Handling Images back to top
Application Notes Western Blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPK3 Antibody Target Details MAPK3 Application Details Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.