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anti-Human MAPK6 antibody for Western Blotting

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • 2610021I23Rik
  • ATMAPK6
  • ATMPK6
  • D130053K17Rik
  • Erk3
  • ERK3
  • ERK6
  • F18O19.10
  • HsT17250
  • Mapk4
  • MAPK6
  • Mapk63
  • MAP KINASE 6
  • MAP kinase 6
  • P38GAMMA
  • p97MAPK
  • Prkm4
  • Prkm6
  • PRKM6
  • PRKM12
  • SAPK-3
  • SAPK3
  • SUSERK3
Epitope
pSer189
25
10
8
8
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
111
56
46
4
3
2
2
2
1
1
1
Host
Rabbit
85
21
12
2
Clonality
Polyclonal
Conjugate
This MAPK6 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
85
66
32
22
11
8
7
6
3
2
1
1
1
Supplier
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Catalog No. ABIN1531591
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN1533932 IF ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1532608 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN969115 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-08 2
0.12303223 ABIN2786700 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN1108134 EIA WB Mouse IgG1 Log in to see 4-00E-008 3
0.12303223 ABIN2452376 IF WB Mouse IgG2a AA 345-721 Log in to see 5E1
0.12303223 ABIN2452531 IHC WB Mouse IgG2a AA 345-721 Log in to see 1A9
0.12303223 ABIN3043611 IHC (p) WB Rabbit IgG AA 520-721 Log in to see Polyclonal
0.12303223 ABIN2706126 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2448661 ELISA WB Rabbit IgG Ser189 Log in to see Polyclonal
0.12303223 ABIN393770 IF WB Mouse IgG2b kappa AA 612-721 Log in to see 4C11
0.12303223 ABIN2448662 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN519218 IF WB Mouse AA 1-721, full length Log in to see Polyclonal
0.12303223 ABIN519221 ELISA WB Mouse IgG2b kappa AA 612-721, partial Log in to see 4C11
0.12303223 ABIN3184549 ELISA WB Rabbit IgG Thr36 Log in to see Polyclonal
0.12303223 ABIN928852 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN519220 IF ELISA WB Mouse IgG2b kappa AA 612-721, partial Log in to see 4C11
0.12303223 ABIN562409 IF RNAi ELISA WB Mouse IgG2a kappa AA 612-721, partial Log in to see 1G6
0.12303223 ABIN1845601 FACS ELISA WB Mouse IgG1 Log in to see
0.12303223 ABIN3181516 WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAPK6 antibody for Western Blotting

Similar anti-MAPK6 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
BioImaging (BI) 1 Antibodies
ELISA 20 Antibodies 29 Antibodies 59 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 9 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 12 Antibodies 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 7 Antibodies
Immunoprecipitation (IP) 2 Antibodies 2 Antibodies
Western Blotting (WB) 36 Antibodies 45 Antibodies
Immunochromatography (IC) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Epitope pSer189
(25), (10), (8), (8), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(111), (56), (46), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(85), (21), (12), (2)
Clonality
Polyclonal
Conjugate This MAPK6 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(85), (66), (32), (22), (11), (8), (7), (6), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN1531591) Images
Specificity ERK3 (Phospho-Ser189) Antibody detects endogenous levels of ERK3 only when phosphorylated at Ser189.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ERK3 around the phosphorylation site of Ser189.
Isotype IgG

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling References for anti-MAPK6 Antibody (ABIN1531591) Images back to top
Antigen
Alternative Name ERK3 (MAPK6 Antibody Abstract)
Background Molecular weight of target protein: 82 kDa
Synonyms: ERK-3, ERK3, Extracellular signal- regulated kinase 3, MAP kinase isoform p97, MAPK6, MK06, Mitogen-activated protein kinase 6, PRKM4, PRKM6, kinase ERK3, p55-MAPK, p97-MAPK
Molecular Weight 82 kDa
Gene ID 5597
OMIM 602904
UniProt Q16659
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling References for anti-MAPK6 Antibody (ABIN1531591) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.411847 (NCBI Gene Symbol: MAPK6)

Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details References for anti-MAPK6 Antibody (ABIN1531591) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAPK6 Antibody (ABIN1531591)

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling Images back to top
Background publications

Greenman, Stephens, Smith, Dalgliesh, Hunter, Bignell, Davies, Teague, Butler, Stevens, Edkins, OMeara, Vastrik, Schmidt, Avis, Barthorpe, Bhamra, Buck, Choudhury, Clements, Cole, Dicks, Forbes, Gray et al.: "Patterns of somatic mutation in human cancer genomes. ..." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007

Meloche, Beatty, Pellerin: "Primary structure, expression and chromosomal locus of a human homolog of rat ERK3." in: Oncogene, Vol. 13, Issue 7, pp. 1575-9, 1996

Images

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN1531591) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (pSer189) (ABIN1531591) Western blot analysis of extracts from mouse brain, using ERK3 (Phospho-Ser189) Antib...
Immunohistochemistry (IHC) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (pSer189) (ABIN1531591) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ERK3...