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|Antigen||Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This MAPK6 antibody is un-conjugated Alternatives|
|Method Type||Peptide ELISA|
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPK6 AntibodyTarget Details MAPK6 Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN2815635|
|Sequence||Peptide sequence: HSPVGSPLKS IQ|
|Cross-Reactivity||Human, Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)|
|Characteristics||Peptide Lenght: 12|
Target Details MAPK6Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
|Alternative Name||ERK3 / MAPK6 (MAPK6 Antibody Abstract)|
|Gene ID||5597, 50772, 58840|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C|
Application DetailsProduct Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug. Western blots of the IP were labelled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 µg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
ImagesProduct Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling back to top