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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||105 Antibodies||3 Antibodies||46 Antibodies||35 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies||17 Antibodies||16 Antibodies|
|Immunohistochemistry (IHC)||41 Antibodies||21 Antibodies||18 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||66 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 7 (MAPK7) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK7 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPK7 AntibodyTarget Details MAPK7 Application Details Handling Images
|Specificity||This antibody recognizes MAP kinase 7 (erk5) at 92 kDa in Western-blot applications.|
|Characteristics||Synonyms: Mitogen-activated protein kinase 7, Extracellular signal-regulated kinase 5, ERK4, Big MAPkinase 1, MAPK7|
|Purification||Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Peptide conjugated to hemocyanin. AA Sequence: N-Terminus|
Target Details MAPK7Product Details anti-MAPK7 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK7 / ERK5 (MAPK7 Antibody Abstract)|
|Background||Erk5 (also known as MAPK7 or big mitogen activated kinase 1, BMK1) belongs to the Ser/Thr protein kinase family. It contains a T E Y motif in the activation loop similar to Erk1/2. Extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxdative stress lead to Erk5 activation by MEK5. The Erk5 pathway plays an important role in cellular proliferation, differentiation and survival.Synonyms: Big MAP kinase 1, ERK4, Extracellular signal-regulated kinase 5, MAPK7, Mitogen-activated protein kinase 7|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-MAPK7 Antibody Target Details MAPK7 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive control: Cell lysate from untreated SKOV-3 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK7 Antibody Target Details MAPK7 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-MAPK7 Antibody Target Details MAPK7 Application Details Handling back to top