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|Antigen||Mitogen-Activated Protein Kinase 9 (MAPK9) Antibodies|
|Epitope||AA 1-424 Alternatives|
|Conjugate||This MAPK9 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), ELISA
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Product Details anti-MAPK9 AntibodyTarget Details MAPK9 Application Details Handling Images
|Specificity||Human JNK2 / MAPK9|
|Characteristics||Produced in rabbits immunized with purified, recombinant Human JNK2 / MAPK9 (rh JNK2 / MAPK9, ABIN2003338 NP_002743.3, Met 1-Arg 424,. JNK2 / MAPK9 specific IgG was purified by Human JNK2 / MAPK9 affinity chromatography.|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human JNK2 proteimn (ABIN2003338)|
Target Details MAPK9Product Details anti-MAPK9 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK9 (MAPK9 Antibody Abstract)|
|Background||Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of MAP kinase subfamily belonging to the protein kinase superfamily. MAPK9 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, such as c-Jun and ATF2. The crystal structure of human JNK2 complexed with an indazole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approach. A novel conformation of the activation loop is observed, which is not compatible with its phosphorylation by upstream kinases. This activation inhibitory conformation of JNK2 is stabilized by the MAP kinase insert that interacts with the activation loop in an induced-fit manner. It suggest that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. JNK2 deficiency leads to reduced c-Jun degradation, thereby augmenting c-Jun levels and cellular proliferation, and suggests that JNK2 is a negative regulator of cellular proliferation in multiple cell types. JNK2 prevents replicative stress by coordinating cell cycle progression and DNA damage repair mechanisms. JNK2 blocks the ubiquitination of tumor suppressor p53, and thus increases the stability of p53 in nonstressed cells. JNK2 negatively regulates antigen-specific CD8+ T cell expansion and effector function, and thus selectively blocking JNK2 in CD8+ T cells may potentially enhance anti-tumor immune response. Lack of JNK2 expression was associated with higher tumor aneuploidy and reduced DNA damage response. Additionally,the JNK2 protein could be a novel therapeutic target in dry eye disease, and may provide a novel target for prevention of vascular disease and atherosclerosis.|
|Pathways||MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAPK9 Antibody Target Details MAPK9 Handling Images back to top
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human MAPK9 . The detection limit for Human MAPK9 is approximately 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK9 Antibody Target Details MAPK9 Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
ImagesProduct Details anti-MAPK9 Antibody Target Details MAPK9 Application Details Handling back to top