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anti-Chicken MAPKAP Kinase 2 antibody for Enzyme Immunoassay

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • AA960234
  • MAPKAP-K2
  • mapkapk2
  • MAPKAPK2
  • MGC78852
  • MK-2
  • MK2
  • Rps6kc1
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
Epitope
AA 310-325
51
24
21
15
13
13
13
11
10
10
8
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
213
135
106
30
4
2
1
1
Host
Rabbit
190
25
20
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
172
103
88
33
32
28
24
18
10
4
1
1
1
1
Supplier
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Catalog No. ABIN117983
$ 500.50
Plus shipping costs $45.00

Top referenced anti-MAPKAP Kinase 2 antibody for Enzyme Immunoassay

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Human Chicken Hamster Mouse (Murine) Rabbit Rat (Rattus) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 153 Antibodies 1 Antibodies 2 Antibodies 97 Antibodies 2 Antibodies 72 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 18 Antibodies 1 Antibodies 2 Antibodies 13 Antibodies 2 Antibodies 7 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope AA 310-325
(51), (24), (21), (15), (13), (13), (13), (11), (10), (10), (8), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(213), (135), (106), (30), (4), (2), (1), (1)
Host Rabbit
(190), (25), (20)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(172), (103), (88), (33), (32), (28), (24), (18), (10), (4), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images
Sequence M-T-S-A-L-A-T-M-R-V-D-Y-E-Q-I-K
Purification Affinity Purified
Immunogen This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Antigen
Alternative Name MAPKAP Kinase-2 (MAPKAPK2 Antibody Abstract)
Background MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2
Gene ID 9986
UniProt P49139
Research Area Signaling, Inflammation, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Application Notes Suitable for use in Western blotting, ELISA, Immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. Affinity purified anti-MAPKAP Kinase 2 is specific for MAPKAPKinase 2 and will not effectively immunoblot other isoforms of the kinase (i. e. MAPKAP K1or K3) from cell lines and/or tissues. Use a HeLa cell lysate as a positive control. MK2assays are often used and are easier and have less background than p38 kinase assays. Immunoprecipitation of MK2 using antibodies is used for this purpose. This product wasassayed by immunoblot and was found to be reactive to 20 g of crude protein extractprepared from HeLa cells. The gel was probed with Affinity Purified anti-MAPKAP Kinase2used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG(H&L) (GOAT) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) as a secondary
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Concentration 0.9 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C. DO NOT FREEZE! This products is photosensitive and should be protected from light.

References for anti-MAPKAP Kinase 2 Antibody (ABIN117983)

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
Product cited in:

Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, 2008

Background publications

Knauf, Mutschler: "[Mechanism of action of xipamide and its classification as a 'low ceiling diuretic'. Pharmacodynamic-pharmacokinetic studies in healthy volunteers and in kidney and liver patients]" in: Arzneimittel-Forschung, Vol. 55, Issue 1, pp. 1-14, 2005

Cheng, Tseng, Chang, Chang, Lai: "Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2." in: Journal of cellular biochemistry, Vol. 89, Issue 3, pp. 589-602, 2003

Neininger, Kontoyiannis, Kotlyarov, Winzen, Eckert, Volk, Holtmann, Kollias, Gaestel: "MK2 targets AU-rich elements and regulates biosynthesis of tumor necrosis factor and interleukin-6 independently at different post-transcriptional levels." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3065-8, 2002

Rousseau, Morrice, Peggie, Campbell, Gaestel, Cohen: "Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs." in: The EMBO journal, Vol. 21, Issue 23, pp. 6505-14, 2002

Verma, Deb, Sassano, Kambhampati, Wickrema, Uddin, Mohindru, Van Besien, Platanias: "Cutting edge: activation of the p38 mitogen-activated protein kinase signaling pathway mediates cytokine-induced hemopoietic suppression in aplastic anemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 5984-8, 2002

Kotlyarov, Neininger, Schubert, Eckert, Birchmeier, Volk, Gaestel: "MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis." in: Nature cell biology, Vol. 1, Issue 2, pp. 94-7, 1999

Zu, Wu, Gilchrist, Ai, Labadia, Huang: "The primary structure of a human MAP kinase activated protein kinase 2." in: Biochemical and biophysical research communications, Vol. 200, Issue 2, pp. 1118-24, 1994

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994

Stokoe, Caudwell, Cohen, Cohen: "The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2." in: The Biochemical journal, Vol. 296 ( Pt 3), pp. 843-9, 1994

Images

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (AA 310-325) (ABIN117983) Affinity purified anti-MAPKAP.,.Kinase 2 polyclonal antibody detects MK2.,.in unstimu...