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|Antigen||Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPKAP Kinase 2 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAPKAP Kinase 2 AntibodyTarget Details MAPKAP Kinase 2 Application Details Handling Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412823|
The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots.
These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.
|Immunogen||Synthetic peptide surrounding amino acid 365 of mouse MAPKAPK-2|
Target Details MAPKAP Kinase 2Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling Images back to top
|Alternative Name||MAPKAPK-2 (MAPKAPK2 Antibody Abstract)|
MAP kinase-activated protein kinase-2 (MAPKAPK-2), also known as p45 hsp27 kinase, is a 45-54 kDa serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MAPKAPK-2 is activated in response to stress, IL-1 and TNF, possibly catalyzed by p38/Hog-dependent phosphorylation. One of the major substrates of MAPKAPK-2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
Synonyms: MAPKAPK2, MK2
|Research Area||Signaling, Inflammation, Kinases/Phosphatases|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling Images back to top
|Application Notes||Western blot analysis (0.5-4 μg/mL) and immunoprecipitation. However, the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit anti-MAPKAPK-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|