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anti-Dog (Canine) MAPKAP Kinase 2 antibody for ELISA

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2
  • MAPKAPK2
  • Mapkapk2
Epitope
AA 339-351
51
24
21
15
15
15
15
11
9
9
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit
222
171
137
43
13
8
7
5
5
4
4
3
2
2
2
1
1
Host
Goat
195
25
23
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
181
96
82
40
39
35
29
17
12
4
4
3
1
1
Supplier
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Catalog No. ABIN1912062
$ 595.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2563872 IP ELISA WB Goat IgG Internal Region Log in to see Polyclonal

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Human Mouse (Murine) Hamster Rabbit Cow (Bovine) Dog (Canine) Pig (Porcine) Guinea Pig Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Western Blotting (WB) 161 Antibodies 6 Antibodies 125 Antibodies 9 Antibodies 20 Antibodies 14 Antibodies 3 Antibodies 4 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 29 Antibodies 4 Antibodies 18 Antibodies 2 Antibodies 8 Antibodies 8 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope AA 339-351
(51), (24), (21), (15), (15), (15), (15), (11), (9), (9), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit
(222), (171), (137), (43), (13), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Goat
(195), (25), (23)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(181), (96), (82), (40), (39), (35), (29), (17), (12), (4), (4), (3), (1), (1)
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling Images
Brand IHC-plus™
Specificity Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-SRVLKEDKERWED from an internal region (near C-terminus) of human MAPKAPK2 / MK2 (NP_004750.1, NP_116584.2). Percent identity by BLAST analysis: Hamster, Panda, Bovine, Dog, Cat, Horse, Rabbit, Pig, Opossum, Guinea pig, Platypus (100%).

Type of Immunogen: Synthetic peptide

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPKAPK2 / MK2 (MAPKAPK2 Antibody Abstract)
Background Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase

Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2
Gene ID 9261
NCBI Accession NP_004750, NP_116584
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling Images back to top
Application Notes Approved: ELISA (1:128000), IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kD band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kD according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec,66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 μg/mL. Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 μg EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.

Images

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (AA 339-351) (ABIN1912062) Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)