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anti-Human MAPKAP Kinase 2 antibody for Intracellular Staining

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • AA960234
  • MAPKAP-K2
  • mapkapk2
  • MAPKAPK2
  • MGC78852
  • MK-2
  • MK2
  • Rps6kc1
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
Epitope
pThr334
50
24
21
15
13
13
13
11
10
10
8
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
213
135
107
30
4
2
2
2
1
1
1
Host
Mouse
191
24
20
Clonality
Monoclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
Application
Intracellular Staining (ICS), Western Blotting (WB)
172
103
88
33
32
28
25
18
10
3
2
1
1
1
Supplier
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Supplier Product No.
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Catalog No. ABIN1177086
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1177089 ICS Alexa Fluor 488 Mouse IgG1, kappa pThr334 Log in to see P24-694 8
0.0008696448 ABIN1177088 ICS Alexa Fluor 647 Mouse IgG1, kappa pThr334 Log in to see P24-694 7
0.0008696448 ABIN1177087 ICS PE Mouse IgG1, kappa pThr334 Log in to see P24-694 8

Top referenced anti-MAPKAP Kinase 2 antibody for Intracellular Staining

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 53 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 10 Antibodies
Immunofluorescence (IF) 13 Antibodies 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Western Blotting (WB) 97 Antibodies
Dot Blot (DB) 18 Antibodies
Functional Studies (Func) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope pThr334
(50), (24), (21), (15), (13), (13), (13), (11), (10), (10), (8), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(213), (135), (107), (30), (4), (2), (2), (2), (1), (1), (1)
Host Mouse
(191), (24), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3)
Application Intracellular Staining (ICS), Western Blotting (WB)
(172), (103), (88), (33), (32), (28), (25), (18), (10), (3), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN1177086) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human MAPKAPK-2 Peptide
Clone P24-694
Isotype IgG1, kappa

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN1177086) Images back to top
Antigen
Alternative Name MAPKAPK-2 (MAPKAPK2 Antibody Abstract)
Background The P24-694 monoclonal antibody specifically binds to the phosphorylated T334 site (pT334) of MAPKAPK-2. MAPKAPK-2 is a serine/threonine protein kinase. This ~49 kDa member of the MAPKAPK family of protein kinases is also known as mitogen-activated protein kinase-activated protein kinase 2. MAPKAPK-2 is phosphorylated and activated by p38 MAP kinase in response to stress, cytokines and chemokines. MAPKAPK-2 is phosphorylated on multiple sites including Thr222, Ser272 and Thr334. Phosphorylation of any two of these three amino acid residues seems to be required for the activation of this kinase that serves multiple cellular functions. Phosphorylation of Thr334 was reported to be essential for nuclear export of the heterodimer formed between p38 MAPK and MAPKAPK-2. Mice deficient in MAPKAPK-2 have been shown to be protected from ischemic injury. MAPKAPK-2 is also reported to serve as a cell cycle checkpoint kinase in response to UV irradiation. The heat shock protein, HSP27 was shown to be one of the major substrates of MAPK and MAPKAPK-2.
Synonyms: MAP kinase-activated protein kinase 2, MAPKAP kinase 2, MK2, MAPK2 Entrez Gene ID: 9261, 17164
Molecular Weight ~49 kDa
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN1177086) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 Antibody (ABIN1177086) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-MAPKAP Kinase 2 Antibody (ABIN1177086)

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
Product cited in:

Manke, Nguyen, Lim, Stewart, Elia, Yaffe: "MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation." in: Molecular cell, Vol. 17, Issue 1, pp. 37-48, 2005

Wang, Xu, Wang, Young, Gaestel, Feuerstein: "Mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 deficiency protects brain from ischemic injury in mice." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43968-72, 2002

Heidenreich, Neininger, Schratt, Zinck, Cahill, Engel, Kotlyarov, Kraft, Kostka, Gaestel, Nordheim: "MAPKAP kinase 2 phosphorylates serum response factor in vitro and in vivo." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14434-43, 1999

Engel, Kotlyarov, Gaestel: "Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation." in: The EMBO journal, Vol. 17, Issue 12, pp. 3363-71, 1998

Krump, Sanghera, Pelech, Furuya, Grinstein: "Chemotactic peptide N-formyl-met-leu-phe activation of p38 mitogen-activated protein kinase (MAPK) and MAPK-activated protein kinase-2 in human neutrophils." in: The Journal of biological chemistry, Vol. 272, Issue 2, pp. 937-44, 1997

Ben-Levy, Leighton, Doza, Attwood, Morrice, Marshall, Cohen: "Identification of novel phosphorylation sites required for activation of MAPKAP kinase-2." in: The EMBO journal, Vol. 14, Issue 23, pp. 5920-30, 1996

Clifton, Young, Cohen: "A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress." in: FEBS letters, Vol. 392, Issue 3, pp. 209-14, 1996

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994