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anti-Human MAPKAP Kinase 2 antibody for Western Blotting

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2
  • MAPKAPK2
  • Mapkapk2
Epitope
AA 310-325
51
22
21
15
15
15
14
9
9
9
8
6
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
218
167
132
43
13
8
7
5
5
5
4
3
3
2
1
1
Host
Rabbit
192
25
22
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
177
97
82
39
39
32
29
17
12
4
3
1
1
1
Supplier
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Catalog No. ABIN117983
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN2788401 IHC WB Rabbit Middle Region Log in to see Polyclonal
3.0923712 ABIN1531440 IHC ELISA WB Rabbit IgG pThr222 Log in to see Polyclonal
3.0923712 ABIN3185456 ELISA IHC WB Rabbit IgG Tyr463 Log in to see Polyclonal
3.0923712 ABIN1531187 IHC ELISA WB Rabbit IgG pThr334 Log in to see Polyclonal
3.0923712 ABIN1532149 IHC ELISA WB Rabbit IgG pSer272 Log in to see Polyclonal
3.0923712 ABIN103960 IP IHC ELISA WB Rabbit IgG AA 310-325 Log in to see Polyclonal
3.0923712 ABIN499033 IHC (p) WB Rabbit Log in to see Polyclonal
3.0923712 ABIN498988 IHC (p) WB Rabbit Log in to see Polyclonal
3.0923712 ABIN1533175 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN1532203 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN1532457 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN4332691 ELISA ICC IF WB Mouse IgG1 kappa pThr2334 Log in to see 9F10-2
3.0923712 ABIN1870393 IF IHC WB Rabbit IgG pThr334 Log in to see Polyclonal
3.0923712 ABIN4332692 IP WB Mouse IgG C-Term Log in to see 7H4-2
3.0923712 ABIN393565 IF WB Mouse IgG2b kappa AA 302-401 Log in to see 2B3
3.0923712 ABIN2463779 IHC WB Rabbit Log in to see Polyclonal
3.0923712 ABIN4332688 ICC IF IHC IHC (p) WB Rabbit pThr334 Log in to see Polyclonal
3.0923712 ABIN408123 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN1859760 ICC IHC IP WB Rabbit IgG AA 139-367 Log in to see Polyclonal
3.0923712 ABIN522743 IF ELISA WB Mouse IgG2b kappa AA 266-352 Log in to see 3A7

Top referenced anti-MAPKAP Kinase 2 antibody for Western Blotting

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Hamster Rabbit Xenopus laevis Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 1 Antibodies
Western Blotting (WB) 157 Antibodies 4 Antibodies 6 Antibodies 121 Antibodies 9 Antibodies 90 Antibodies 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 27 Antibodies 1 Antibodies 4 Antibodies 16 Antibodies 2 Antibodies 10 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope AA 310-325
(51), (22), (21), (15), (15), (15), (14), (9), (9), (9), (8), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(218), (167), (132), (43), (13), (8), (7), (5), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(192), (25), (22)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(177), (97), (82), (39), (39), (32), (29), (17), (12), (4), (3), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images
Sequence M-T-S-A-L-A-T-M-R-V-D-Y-E-Q-I-K
Purification Affinity Purified
Immunogen This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to aa 310-325 of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Antigen
Alternative Name MAPKAP Kinase-2 (MAPKAPK2 Antibody Abstract)
Background MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-α at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2
Gene ID 9986
UniProt P49139
Research Area Signaling, Inflammation, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Application Notes Suitable for use in Western blotting, ELISA, Immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. Affinity purified anti-MAPKAP Kinase 2 is specific for MAPKAPKinase 2 and will not effectively immunoblot other isoforms of the kinase (i. e. MAPKAP K1or K3) from cell lines and/or tissues. Use a HeLa cell lysate as a positive control. MK2assays are often used and are easier and have less background than p38 kinase assays. Immunoprecipitation of MK2 using antibodies is used for this purpose. This product wasassayed by immunoblot and was found to be reactive to 20 g of crude protein extractprepared from HeLa cells. The gel was probed with Affinity Purified anti-MAPKAP Kinase2used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG(H&L) (GOAT) (Min X Bv Ch Gt GP Hs Hu Ms Rt & Sh Serum Proteins) as a secondary
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) Images back to top
Concentration 0.9 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C. DO NOT FREEZE! This products is photosensitive and should be protected from light.

References for anti-MAPKAP Kinase 2 Antibody (ABIN117983)

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
Product cited in:

Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, 2008

Background publications

Knauf, Mutschler: "[Mechanism of action of xipamide and its classification as a 'low ceiling diuretic'. Pharmacodynamic-pharmacokinetic studies in healthy volunteers and in kidney and liver patients]" in: Arzneimittel-Forschung, Vol. 55, Issue 1, pp. 1-14, 2005

Cheng, Tseng, Chang, Chang, Lai: "Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2." in: Journal of cellular biochemistry, Vol. 89, Issue 3, pp. 589-602, 2003

Neininger, Kontoyiannis, Kotlyarov, Winzen, Eckert, Volk, Holtmann, Kollias, Gaestel: "MK2 targets AU-rich elements and regulates biosynthesis of tumor necrosis factor and interleukin-6 independently at different post-transcriptional levels." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3065-8, 2002

Rousseau, Morrice, Peggie, Campbell, Gaestel, Cohen: "Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs." in: The EMBO journal, Vol. 21, Issue 23, pp. 6505-14, 2002

Verma, Deb, Sassano, Kambhampati, Wickrema, Uddin, Mohindru, Van Besien, Platanias: "Cutting edge: activation of the p38 mitogen-activated protein kinase signaling pathway mediates cytokine-induced hemopoietic suppression in aplastic anemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 5984-8, 2002

Kotlyarov, Neininger, Schubert, Eckert, Birchmeier, Volk, Gaestel: "MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis." in: Nature cell biology, Vol. 1, Issue 2, pp. 94-7, 1999

Zu, Wu, Gilchrist, Ai, Labadia, Huang: "The primary structure of a human MAP kinase activated protein kinase 2." in: Biochemical and biophysical research communications, Vol. 200, Issue 2, pp. 1118-24, 1994

Rouse, Cohen, Trigon, Morange, Alonso-Llamazares, Zamanillo, Hunt, Nebreda: "A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins." in: Cell, Vol. 78, Issue 6, pp. 1027-37, 1994

Stokoe, Caudwell, Cohen, Cohen: "The substrate specificity and structure of mitogen-activated protein (MAP) kinase-activated protein kinase-2." in: The Biochemical journal, Vol. 296 ( Pt 3), pp. 843-9, 1994

Images

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN117983) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (AA 310-325) (ABIN117983) Affinity purified anti-MAPKAP.,.Kinase 2 polyclonal antibody detects MK2.,.in unstimu...