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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||55 Antibodies||23 Antibodies||9 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||6 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||23 Antibodies||11 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||4 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||1 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPKAP Kinase 3 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAPKAP Kinase 3 AntibodyTarget Details MAPKAP Kinase 3 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of 3pK protein. (region surrounding Arg319)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details MAPKAP Kinase 3Product Details anti-MAPKAP Kinase 3 Antibody Application Details Handling Images back to top
|Alternative Name||MAPKAP Kinase-3 (MAPKAPK3 Antibody Abstract)|
|Background||The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK 1 and ERK 2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAPK-2 and MAPKAPK-3 (also designated 3pK). The serine/threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases.Synonyms: MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAPK-3, MAPKAPK3|
|Molecular Weight||approx. 42 kDa|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-MAPKAP Kinase 3 Antibody Target Details MAPKAP Kinase 3 Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPKAP Kinase 3 Antibody Target Details MAPKAP Kinase 3 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-MAPKAP Kinase 3 Antibody Target Details MAPKAP Kinase 3 Application Details Handling back to top