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anti-Human MAPKAP Kinase 5 antibody for Western Blotting

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAP-K5
  • MAPKAPK5
  • MK-5
  • MK5
  • PRAK
Epitope
Thr182
23
21
20
14
13
8
7
6
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
143
59
7
2
2
2
Host
Rabbit
98
42
20
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
135
86
39
12
12
9
9
2
1
1
1
Supplier
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Catalog No. ABIN359410
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12226487 ABIN389587 IHC (p) WB Rabbit Ig pSer93 Log in to see Polyclonal 1
0.12226487 ABIN969362 IHC ELISA WB Mouse IgG1 Log in to see 7H10B4 3
0.12226487 ABIN2856185 WB Rabbit IgG Center Log in to see Polyclonal
0.12226487 ABIN2856547 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.12226487 ABIN391320 WB Rabbit Ig AA 160-189, Thr182 Log in to see Polyclonal
0.12226487 ABIN1532150 IHC ELISA WB Rabbit IgG pThr182 Log in to see Polyclonal
0.12226487 ABIN1108138 IHC (p) WB Mouse IgG1 Log in to see 7H10B4 3
0.12226487 ABIN2464093 IHC ELISA WB Mouse IgG1 truncated Log in to see 7H10B4
0.12226487 ABIN2447988 ELISA WB Rabbit IgG Thr182 Log in to see Polyclonal
0.12226487 ABIN931334 IHC ELISA WB Mouse IgG1 Log in to see 7H10B4
0.12226487 ABIN948701 IP WB Rabbit AA 1-471, full length Log in to see Polyclonal
0.12226487 ABIN522000 WB Mouse AA 1-471, full length Log in to see Polyclonal
0.12226487 ABIN2445379 IF WB Rabbit IgG Log in to see Polyclonal
0.12226487 ABIN522004 IF ELISA WB Mouse IgG1 kappa AA 371-471, partial Log in to see 3D7
0.12226487 ABIN522003 ELISA WB Mouse IgG1 kappa AA 371-471, partial Log in to see 2D5
0.12226487 ABIN2447989 IHC WB Rabbit Ig Fraction pSer93 Log in to see Polyclonal
0.12226487 ABIN522001 WB Mouse AA 1-471, full length Log in to see Polyclonal
0.12226487 ABIN522002 PLA WB Rabbit AA 1-471, full length Log in to see Polyclonal
0.12226487 ABIN1845621 IHC WB Rabbit pThr182 Log in to see Polyclonal
0.12226487 ABIN1844770 IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-MAPKAP Kinase 5 antibody for Western Blotting

Similar anti-MAPKAP Kinase 5 Antibodies

Application / Reactivity Human
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 122 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Epitope Thr182
(23), (21), (20), (14), (13), (8), (7), (6), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(143), (59), (7), (2), (2), (2)
Host Rabbit
(98), (42), (20)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(135), (86), (39), (12), (12), (9), (9), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN359410) Images
Specificity This antibody detects PRAK (MAPKAPK5).
Purification Protein A column, followed by peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T182 of human MAPKAPK5.
Isotype Ig

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN359410) Images back to top
Antigen
Alternative Name MAPKAP Kinase-5 (MAPKAPK5 Antibody Abstract)
Background MAPKAPK5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.Synonyms: MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
Molecular Weight 54220 Da
Gene ID 8550, 9606
UniProt Q8IW41
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN359410) Images back to top
Application Notes ELISA 1: 1,000. Western blott 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details References for anti-MAPKAP Kinase 5 Antibody (ABIN359410) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-MAPKAP Kinase 5 Antibody (ABIN359410)

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling Images back to top
Background publications

Sudo, Maruyama, Osada: "p62 functions as a p38 MAP kinase regulator." in: Biochemical and biophysical research communications, Vol. 269, Issue 2, pp. 521-5, 2000

New, Jiang, Zhao, Liu, Zhu, Flood, Kato, Parry, Han: "PRAK, a novel protein kinase regulated by the p38 MAP kinase." in: The EMBO journal, Vol. 17, Issue 12, pp. 3372-84, 1998

Ni, Wang, Diener, Yao: "MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase." in: Biochemical and biophysical research communications, Vol. 243, Issue 2, pp. 492-6, 1998

Images

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN359410) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (Thr182) (ABIN359410) Western blot analysis of MAPKAPK5 Antibody in Hela cell line lysates (35 µg/lane). MA...