anti-Pig (Porcine) MAPKAP Kinase 5 antibody for Immunoprecipitation

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAPK5
  • MK5
  • PRAK
  • MAPKAP-K5
  • MK-5
  • mitogen-activated protein kinase-activated protein kinase 5
  • MAP kinase-activated protein kinase 5
  • MAPKAPK5
  • mapkapk5
  • Mapkapk5
Epitope
AA 192-206, Internal Region
33
20
19
15
12
10
6
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
160
79
14
7
6
6
3
3
3
3
3
2
2
Host
Goat
106
47
23
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
151
87
40
22
14
12
11
4
3
1
1
1
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Catalog No. ABIN5541352
$ 442.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5541351 EIA IHC (p) IP WB Goat Internal Region Log in to see Polyclonal
1 ABIN2563873 IP ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN2563874 IP ELISA WB Goat IgG AA 192-206 Log in to see Polyclonal
1 ABIN1912072 IF IP IHC (p) WB Rabbit IgG AA 423-473 Log in to see Polyclonal

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Epitope AA 192-206, Internal Region
(33), (20), (19), (15), (12), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(160), (79), (14), (7), (6), (6), (3), (3), (3), (3), (3), (2), (2)
Host Goat
(106), (47), (23)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(151), (87), (40), (22), (14), (12), (11), (4), (3), (1), (1), (1)
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling Images
Sequence C-PQVLEAQRRH QKEKS
Specificity Recognizes MK5 / MAPKAPK5 (192-206).
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region (192-206) of the protein sequence according to NP_003659.2 NP_620777.1.

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name mapkap kinase-5 (MAPKAPK5 Antibody Abstract)
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling Images back to top
Application Notes Peptide ELISA: 1/64000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP only the expected band was observed in the MK5 lane after AP32809PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg AP32808PU-N or AP32809PU-N. Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55 kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Immunohistochemistry on Paraffin Sections: 4-8 μg/mL. In paraffin embedded Human Kidney shows nuclear staining in the DCT.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Images back to top
Format Liquid
Buffer Tris saline, pH ~7.3, 0 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months