anti-Monkey MCL-1 antibody for Immunohistochemistry

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
  • myeloid cell leukemia sequence 1
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • Mcl1
  • MCL1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
330
126
82
13
13
9
8
3
3
3
2
1
1
1
Host
Rabbit
219
97
22
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
6
5
5
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
281
141
116
92
80
45
31
28
26
25
6
5
5
1
Options
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Catalog No. ABIN222889
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN285856 IP IHC WB Rabbit Log in to see Polyclonal
1 ABIN4965419 IHC IP WB Rabbit IgG Log in to see Polyclonal

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(330), (126), (82), (13), (13), (9), (8), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(219), (97), (22), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(281), (141), (116), (92), (80), (45), (31), (28), (26), (25), (6), (5), (5), (1)
Pubmed 2 references available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN222889) Images
Blocking Peptide Blocking peptide for this product available: ABIN412827
Specificity Detects 43 kDa Mcl-1
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN222889) Images back to top
Antigen
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Synonyms: MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT
Gene ID 4170
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling ProductDetails: References for anti-MCL-1 antibody (ABIN222889) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually.
Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details ProductDetails: References for anti-MCL-1 antibody (ABIN222889) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

ProductDetails: References for anti-MCL-1 antibody (ABIN222889)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Product cited in:

Xu, Wells, Padilla, Kato, Kim, Lin: "A signaling pathway consisting of miR-551b, catalase and MUC1 contributes to acquired apoptosis resistance and chemoresistance." in: Carcinogenesis, Vol. 35, Issue 11, pp. 2457-66, 2014

Wang, Chen, Zeng, Bai, Tesfaigzi, Belinsky, Lin: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN222889) back to top
Supplier Images
Western Blotting (WB) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) antibody (ABIN222889) Western blot analysis of Mcl-1 in Ramos cell lysate.