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|Antigen||Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies|
|Reactivity||Cat (Feline), Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This MCL-1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-MCL-1 AntibodyTarget Details MCL-1 Application Details Handling Images
|Specificity||This antibody detects Mcl-1.|
|Immunogen||Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1|
Target Details MCL-1Product Details anti-MCL-1 Antibody Application Details Handling Images back to top
|Alternative Name||MCL1 (MCL1 Antibody Abstract)|
|Background||Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004|
|Research Area||Cancer, Apoptosis/Necrosis|
Application DetailsProduct Details anti-MCL-1 Antibody Target Details MCL-1 Handling Images back to top
Western Blot (0.5-4 μg/mL). Immunoprecipitation (10-20 μg/mL). Immunohistochemistry (10-20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Images back to top
|Buffer||PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.|
ImagesProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling back to top