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|Application / Reactivity||Human||Monkey||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||274 Antibodies||5 Antibodies||72 Antibodies||50 Antibodies|
|Immunoprecipitation (IP)||26 Antibodies||3 Antibodies||9 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||86 Antibodies||1 Antibodies||17 Antibodies||14 Antibodies|
|Immunohistochemistry (IHC)||116 Antibodies||3 Antibodies||23 Antibodies||21 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Antigen||Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MCL-1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-MCL-1 AntibodyTarget Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412827|
|Specificity||Detects 43 kDa Mcl-1|
|Immunogen||Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1|
Target Details MCL-1Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
|Alternative Name||Mcl-1 (MCL1 Antibody Abstract)|
Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Synonyms: MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT
|Research Area||Cancer, Apoptosis/Necrosis|
Application DetailsProduct Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN222889) Images back to top
|Buffer||100 μg (0.2 mg/mL) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-MCL-1 Antibody (ABIN222889)Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
|Product cited in:||
Xu, Wells, Padilla, Kato, Kim, Lin: "A signaling pathway consisting of miR-551b, catalase and MUC1 contributes to acquired apoptosis resistance and chemoresistance." in: Carcinogenesis, Vol. 35, Issue 11, pp. 2457-66, 2014
Wang, Chen, Zeng, Bai, Tesfaigzi, Belinsky, Lin: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008
ImagesProduct Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) back to top