anti-Mouse (Murine) MCL-1 antibody for Western Blotting

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
  • myeloid cell leukemia sequence 1
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • Mcl1
  • MCL1
Epitope
AA 191-226, BH3 Domain
31
22
18
17
15
15
14
13
12
8
6
6
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
324
125
82
13
13
10
8
3
3
3
2
1
1
1
Host
Rabbit
214
96
22
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
6
5
5
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
276
137
115
90
78
44
30
30
26
25
6
5
5
1
Options
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Catalog No. ABIN388105
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1455724 ABIN185242 ELISA WB Goat C-Term Log in to see Polyclonal 1
3.1455724 ABIN222889 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
3.1455724 ABIN3043876 IHC (p) WB Rabbit IgG AA 1-350 Log in to see Polyclonal
3.1455724 ABIN675369 IF (p) IHC (p) WB Rabbit IgG AA 210-248 Log in to see Polyclonal 4
3.1455724 ABIN4333161 ELISA WB Goat C-Term Log in to see Polyclonal 1
3.1455724 ABIN3187703 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1455724 ABIN3185490 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1455724 ABIN1532715 ELISA WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN1533338 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN2465932 ELISA WB Goat C-Term Log in to see Polyclonal
3.1455724 ABIN2801930 WB Rabbit IgG Log in to see Polyclonal 5
3.1455724 ABIN1873660 WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN4333174 ELISA ICC IF WB Rabbit Log in to see Polyclonal
3.1455724 ABIN3020788 WB Rabbit IgG C-Term Log in to see Polyclonal
3.1455724 ABIN4333168 ELISA WB Goat C-Term Log in to see Polyclonal
3.1455724 ABIN3020985 IHC WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN2475515 ELISA WB Goat IgG C-Term Log in to see Polyclonal
3.1455724 ABIN1869118 ICC IHC IP WB Rabbit IgG AA 2-307 Log in to see Polyclonal
3.1455724 ABIN4333155 WB Rabbit Log in to see Polyclonal
3.1455724 ABIN2563805 ELISA WB Goat IgG C-Term Log in to see Polyclonal

Top referenced anti-MCL-1 antibody for Western Blotting

Similar anti-MCL-1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 46 Antibodies 130 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 6 Antibodies
Flow Cytometry (FACS) 14 Antibodies 31 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 43 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 87 Antibodies
Immunochromatography (IC) 5 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope AA 191-226, BH3 Domain
(31), (22), (18), (17), (15), (15), (14), (13), (12), (8), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(324), (125), (82), (13), (13), (10), (8), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(214), (96), (22), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(276), (137), (115), (90), (78), (44), (30), (30), (26), (25), (6), (5), (5), (1)
Pubmed 5 references available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN388105) Images
Specificity This MCL1 antibody was developed against a synthetic peptide selected from the central region (BH3 domain) of human Mcl-1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-226 AA from human MCL1.
Clone RB00961
Isotype Ig

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN388105) Images back to top
Antigen
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Synonyms: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-related protein EAT/mcl1, mcl1/EAT, MCL1
Molecular Weight 37323 Da
Gene ID 4170
UniProt Q07820
Research Area Apoptosis/Necrosis, Cancer, Cell Structure
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling ProductDetails: References for anti-MCL-1 antibody (ABIN388105) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details ProductDetails: References for anti-MCL-1 antibody (ABIN388105) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-MCL-1 antibody (ABIN388105)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Product cited in:

Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, 2012

Tsang, Tang, Chan, Co, Au Yeung, Yau, Kong, Fung, Kwok: "P-glycoprotein enhances radiation-induced apoptotic cell death through the regulation of miR-16 and Bcl-2 expressions in hepatocellular carcinoma cells." in: Apoptosis : an international journal on programmed cell death, Vol. 16, Issue 5, pp. 524-35, 2011

Johnson, Lucas, Muthusamy, Smith, Edwards, De Lay, Croce, Grever, Byrd: "Characterization of the TCL-1 transgenic mouse as a preclinical drug development tool for human chronic lymphocytic leukemia." in: Blood, Vol. 108, Issue 4, pp. 1334-8, 2006

Yamane, Dylla, Muijtjens, Weissman: "Enforced Bcl-2 expression overrides serum and feeder cell requirements for mouse embryonic stem cell self-renewal." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 9, pp. 3312-7, 2005

Background publications

Nijhawan, Fang, Traer, Zhong, Gao, Du, Wang: "Elimination of Mcl-1 is required for the initiation of apoptosis following ultraviolet irradiation." in: Genes & development, Vol. 17, Issue 12, pp. 1475-86, 2003

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling ProductDetails: References for anti-MCL-1 antibody (ABIN388105) back to top
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 191-226) (ABIN388105) Western blot analysis of anti-Mcl-1 BH3 Domain Pab (ABIN388105) in Ramos cell line ly...
Immunohistochemistry (IHC) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 191-226) (ABIN388105) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Immunohistochemistry (IHC) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 191-226) (ABIN388105) Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 D...