anti-Mouse (Murine) MCL-1 antibody for Western Blotting

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • Mcl-1
  • BCL2L3
  • EAT
  • MCL1-ES
  • MCL1L
  • MCL1S
  • TM
  • bcl2-L-3
  • mcl1/EAT
  • mcl-1
  • myeloid cell leukemia sequence 1
  • myeloid cell leukemia sequence 1 (BCL2-related)
  • Mcl1
  • MCL1
Epitope
AA 1-350
31
22
19
17
15
15
14
13
11
9
7
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
330
126
82
13
13
10
8
3
3
3
2
1
1
1
Host
Rabbit
219
97
22
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
6
5
5
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
281
141
117
91
80
45
31
29
26
25
6
5
5
1
Options
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Catalog No. ABIN3043876
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9060245 ABIN675369 IF (p) IHC (p) WB Rabbit IgG AA 210-248 Log in to see Polyclonal 4
2.9060245 ABIN388105 FACS IHC (p) WB Rabbit Ig AA 191-226, BH3 Domain Log in to see Polyclonal 5
2.9060245 ABIN222889 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
2.9060245 ABIN185242 ELISA WB Goat C-Term Log in to see Polyclonal 1
2.9060245 ABIN1532715 ELISA WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN4333161 ELISA WB Goat C-Term Log in to see Polyclonal 1
2.9060245 ABIN3185490 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9060245 ABIN3187703 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9060245 ABIN1533338 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN1869118 ICC IHC IP WB Rabbit IgG AA 2-307 Log in to see Polyclonal
2.9060245 ABIN2801930 WB Rabbit IgG Log in to see Polyclonal 5
2.9060245 ABIN2465932 ELISA WB Goat C-Term Log in to see Polyclonal
2.9060245 ABIN2475515 ELISA WB Goat IgG C-Term Log in to see Polyclonal
2.9060245 ABIN3020788 WB Rabbit IgG C-Term Log in to see Polyclonal
2.9060245 ABIN4333174 ELISA ICC IF WB Rabbit Log in to see Polyclonal
2.9060245 ABIN1873660 WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN3020985 IHC WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN4333168 ELISA WB Goat C-Term Log in to see Polyclonal
2.9060245 ABIN2563805 ELISA WB Goat IgG C-Term Log in to see Polyclonal
2.9060245 ABIN3031299 FACS IHC ELISA WB Rabbit Ig Fraction AA 191-226 Log in to see Polyclonal

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope AA 1-350
(31), (22), (19), (17), (15), (15), (14), (13), (11), (9), (7), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(330), (126), (82), (13), (13), (10), (8), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(219), (97), (22), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(281), (141), (117), (91), (80), (45), (31), (29), (26), (25), (6), (5), (5), (1)
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myeloid cell leukemia 1
Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
Purification Immunogen affinity purified.
Immunogen E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

Synonyms: Bcl 2 related protein EAT/mcl1 antibody|Bcl-2-like protein 3 antibody|Bcl-2-related protein EAT/mcl1 antibody|BCL2 related antibody|Bcl2-L-3 antibody|BCL2L3 antibody|EAT antibody|Induced myeloid leukemia cell differentiation protein Mcl 1 antibody|Induced myeloid leukemia cell differentiation protein Mcl-1 antibody|MCL 1 antibody|MCL1 antibody|MCL1-ES antibody|Mcl1/EAT antibody|MCL1_HUMAN antibody|MCL1L antibody|MCL1S antibody|MGC104264 antibody|MGC1839 antibody|Myeloid Cell Leukemia 1 antibody|Myeloid cell leukemia ES antibody|myeloid cell leukemia sequence 1 antibody|Myeloid cell leukemia sequence 1 BCL2 related antibody|Myeloid cell leukemia sequence 1 isoform 1 antibody|OTTHUMP00000032794 antibody|OTTHUMP00000032795 antibody|TM antibody
Gene ID 4170
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for MCL1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody (ABIN3043876) Anti-MCL1 Picoband antibody, All lanes: Anti MCL1 at 0.5ug/ml WB: Recombinant Human...
Immunohistochemistry (IHC) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody (ABIN3043876) Anti-MCL1 Picoband antibody, IHC(P): Human Intestinal Cancer Tissue
Immunohistochemistry (IHC) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody (ABIN3043876) Anti-MCL1 Picoband antibody, IHC(P): Rat Intestine Tissue
Immunohistochemistry (IHC) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody (ABIN3043876) Anti-MCL1 Picoband antibody, IHC(P): Mouse Intestine Tissue
Western Blotting (WB) image for anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 1-350) antibody (ABIN3043876) Observed bind size: 37KD