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anti-Mouse (Murine) MCL-1 antibody for Western Blotting

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
297
101
67
9
8
4
4
3
2
2
1
1
Host
Rabbit
205
82
18
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
7
6
5
3
2
2
2
2
2
2
2
2
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
250
123
102
64
60
31
31
22
20
13
7
6
1
Supplier
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Catalog No. ABIN222889
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12650052 ABIN500252 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.12650052 ABIN185242 ELISA WB Goat C-Term Log in to see Polyclonal 1
0.12650052 ABIN388105 FACS IHC (p) WB Rabbit Ig AA 191-226, BH3 Domain Log in to see Polyclonal 5
0.12650052 ABIN675369 IF (p) IHC (p) WB Rabbit IgG AA 210-248 Log in to see Polyclonal 3
0.12650052 ABIN1533338 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN1532715 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN3043876 IHC (p) WB Rabbit IgG AA 1-350 Log in to see Polyclonal
0.12650052 ABIN2465932 ELISA WB Goat C-Term Log in to see Polyclonal
0.12650052 ABIN2801930 WB Rabbit IgG Log in to see Polyclonal 5
0.12650052 ABIN2448022 ELISA WB Rabbit IgG Ser159 Log in to see Polyclonal
0.12650052 ABIN1873660 WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN1873661 IHC WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN3020788 WB Rabbit IgG C-Term Log in to see Polyclonal
0.12650052 ABIN2448778 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN3020985 IHC WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN1869118 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2-307 Log in to see Polyclonal
0.12650052 ABIN3187703 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12650052 ABIN3185490 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12650052 ABIN1030511 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.12650052 ABIN2403506 WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MCL-1 antibody for Western Blotting

Similar anti-MCL-1 Antibodies

Application / Reactivity Human Monkey Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 245 Antibodies 5 Antibodies 63 Antibodies 45 Antibodies
Immunoprecipitation (IP) 21 Antibodies 3 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies 1 Antibodies 15 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 103 Antibodies 3 Antibodies 21 Antibodies 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(297), (101), (67), (9), (8), (4), (4), (3), (2), (2), (1), (1)
Host Rabbit
(205), (82), (18), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (7), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(250), (123), (102), (64), (60), (31), (31), (22), (20), (13), (7), (6), (1)
Pubmed 2 references available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images
Blocking Peptide Blocking peptide for this product available: ABIN412827
Specificity Detects 43 kDa Mcl-1
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Antigen
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Synonyms: MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT
Gene ID 4170
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-MCL-1 Antibody (ABIN222889)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Product cited in:

Xu, Wells, Padilla, Kato, Kim, Lin: "A signaling pathway consisting of miR-551b, catalase and MUC1 contributes to acquired apoptosis resistance and chemoresistance." in: Carcinogenesis, Vol. 35, Issue 11, pp. 2457-66, 2014

Wang, Chen, Zeng, Bai, Tesfaigzi, Belinsky, Lin: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) back to top
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (ABIN222889) Western blot analysis of Mcl-1 in Ramos cell lysate.