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anti-Human PAK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Epitope
pThr402, pThr423
55
26
24
16
13
10
10
10
10
9
7
7
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
320
153
149
4
4
1
1
Host
Rabbit
289
17
16
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
227
127
126
58
53
44
22
22
16
7
6
4
1
Supplier
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Catalog No. ABIN498797
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498954 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN498222 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN499018 IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN498888 IHC (p) WB Rabbit Log in to see Polyclonal
0.34288716 ABIN615840 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN360317 EIA IHC (p) WB Rabbit Ig Thr423 Log in to see Polyclonal 3
0.0008982679 ABIN392432 IF IHC (p) WB Rabbit Ig AA 401-430, Thr423 Log in to see Polyclonal 1
0.0008982679 ABIN197364 IF IHC (p) WB Rabbit Thr212 Log in to see Polyclonal 3
0.0008982679 ABIN197378 IF IHC (p) Rabbit Thr423 Log in to see Polyclonal 4
0.0008982679 ABIN196878 IF IHC (p) WB Rabbit pThr212 Log in to see Polyclonal 3
0.0008982679 ABIN196896 IF IHC (p) Rabbit pThr402, pThr421, pThr423 Log in to see Polyclonal 4
0.0008982679 ABIN272055 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN272054 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN562102 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 191-280, partial Log in to see 4D1 1
0.0008982679 ABIN782427 EIA IHC (p) WB Mouse IgG1 AA 191-280 Log in to see 1-00E-011
0.0008982679 ABIN2879047 IF IHC (p) ELISA WB Rabbit IgG AA 165-214 Log in to see Polyclonal
0.0008982679 ABIN2879005 IF IHC (p) ELISA WB Rabbit IgG AA 178-227 Log in to see Polyclonal
0.0008982679 ABIN373751 IHC (p) WB Rabbit AA 525-545 Log in to see Polyclonal
0.0008982679 ABIN1688582 IHC (p) Rabbit IgG AA 525-545 Log in to see Polyclonal
0.0008982679 ABIN2855705 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal

Top referenced anti-PAK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PAK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 229 Antibodies 103 Antibodies 99 Antibodies
Immunoprecipitation (IP) 23 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies 32 Antibodies 32 Antibodies
Immunohistochemistry (IHC) 125 Antibodies 71 Antibodies 70 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies 44 Antibodies
Immunofluorescence (IF) 60 Antibodies 39 Antibodies 39 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope pThr402, pThr423
(55), (26), (24), (16), (13), (10), (10), (10), (10), (9), (7), (7), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(320), (153), (149), (4), (4), (1), (1)
Host Rabbit
(289), (17), (16), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(227), (127), (126), (58), (53), (44), (22), (22), (16), (7), (6), (4), (1)
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling Images
Specificity This antibody detects endogenous levels of p-PAK1/2 protein, only when phosphorylated at Thr423/402.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background Three isoforms of Serine/Threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. The site of autophosphorylation on αPAK is Thr 423, which is in the kinase activation loop. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK-4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac 1 and Cdc42, they do not interact with Rho.Synonyms: Alpha-PAK, Gamma-PAK, PAK 1, PAK 2, PAK alpha, PAK gamma, PAK-1, PAK-2, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, p21-activated kinase 1, p21-activated kinase 2, p58, p65-PAK
Molecular Weight approx. 60 kDa
Gene ID 5058
NCBI Accession NP_001122092
UniProt Q13153
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
Application Notes Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PAK1 antibody (P21-Activated Kinase 1) (pThr402) (ABIN498797) Immunohistochemistry (IHC) analyzes of p-PAK1/2 (pThr423/402) antibody in para...