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anti-Mouse (Murine) PAK1 antibody for Immunoprecipitation

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Epitope
AA 484-502
55
26
24
17
13
10
10
10
9
9
7
7
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
314
148
145
4
4
1
1
Host
Rabbit
284
17
15
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Immunoprecipitation (IP), Western Blotting (WB)
220
125
124
55
51
44
22
21
13
7
6
4
1
Supplier
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Catalog No. ABIN493209
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235196 ABIN189638 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see EP795Y
0.00089235196 ABIN1874025 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN2405452 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN2973990 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-PAK1 antibody for Immunoprecipitation

Similar anti-PAK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 224 Antibodies 98 Antibodies 95 Antibodies
Immunoprecipitation (IP) 23 Antibodies 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies 29 Antibodies 29 Antibodies
Immunohistochemistry (IHC) 122 Antibodies 68 Antibodies 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies 44 Antibodies
Immunofluorescence (IF) 57 Antibodies 36 Antibodies 36 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope AA 484-502
(55), (26), (24), (17), (13), (10), (10), (10), (9), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(314), (148), (145), (4), (4), (1), (1)
Host Rabbit
(284), (17), (15), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunoprecipitation (IP), Western Blotting (WB)
(220), (125), (124), (55), (51), (44), (22), (21), (13), (7), (6), (4), (1)
Pubmed 3 references available
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN493209) Images
Specificity This antibody detects a single band of approximately 65 kDa of PAK in Hek 293 cell extracts or in Western blot positive control for PAK samples. The antibody does not cross react with other proteins from the protein kinase family or with other proteins.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography
Immunogen Synthetic peptide form lymphocyte p21 PAK65kinase corresponding to position 484-502

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 Antibody (ABIN493209) Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background A brain serine threonine protein kinase is a target for p21 ras related proteins Cdc42 and Rac1. p21-activated kinases (PAKs) bind to and are activated by Rho family GTPases such as Cdc42 and Rac. These GTPases play key roles in regulating cell polarity, stress responses, and cell cycle progression (1). Stiochiometeric analyses using purified Rac and PAK suggest that Rac binds to PAK with high affinity in a 1:1 ratio. The kinase complexes specifically with aGTP bound activated p21, inhibiting the p21 activity and leading to activation and auto-phosphorylation of p65pak (2). Three isoforms of p21-activated serine/threonine kinases, PAK68, PAK65 and PAK62, were found in both cytosolic & membrane fraction, & their contents were significantly increased during induced retinoic acid differentiation of human promyelocytic leukemia HL60 cell (3). The content of PAK65 or Rac is not evident in plasma membrane fraction of cells form un-differentiated cells (2). Constitutively activated Pak1 also suppress the activity of scavenger receptor class B, type 1 (SB-B1) in macrophage cell line RAW264.7, an action similar to LPS (4). Mutational studies with PAK1 also suggest that SR-B1 receptor down regulation involves binding to Rac and Cdc42. In mammalian cells, hyper osmotic shocks can induce apoptotic cell death. It is suggested that hyper osmotic shock can induce cleavage for PAK2 to produce a 36 kDa kinase, which is the result of DNA fragmentation and activation of ICE/CED-3 family cysteine proteases termed caspases-3.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK
Gene ID 5058
NCBI Accession NP_001122092
UniProt Q13153
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 Antibody (ABIN493209) Images back to top
Application Notes IMM and i. p pull-down assays: > 1: 100. Western blot: > 1: 250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 Antibody (ABIN493209) Images back to top
Concentration 1.0 - 1.25 mg/mL
Buffer Stabilization buffer with 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-PAK1 Antibody (ABIN493209)

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Background publications

Nisimoto, Ogawa: "Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid." in: European journal of biochemistry / FEBS, Vol. 269, Issue 10, pp. 2622-9, 2002

Hullinger, Panek, Xu, Karathanasis: "p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 46807-14, 2001

Sells, Barratt, Caviston, Ottilie, Leberer, Chernoff: "Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18490-8, 1998