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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||224 Antibodies||98 Antibodies||95 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||53 Antibodies||29 Antibodies||29 Antibodies|
|Immunohistochemistry (IHC)||122 Antibodies||68 Antibodies||67 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||44 Antibodies||44 Antibodies||44 Antibodies|
|Immunofluorescence (IF)||57 Antibodies||36 Antibodies||36 Antibodies|
|Antigen||P21-Activated Kinase 1 (PAK1) Antibodies|
|Epitope||AA 484-502 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PAK1 AntibodyTarget Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN493209) Images
|Specificity||This antibody detects a single band of approximately 65 kDa of PAK in Hek 293 cell extracts or in Western blot positive control for PAK samples. The antibody does not cross react with other proteins from the protein kinase family or with other proteins.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Immunogen||Synthetic peptide form lymphocyte p21 PAK65kinase corresponding to position 484-502|
Target Details PAK1Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 Antibody (ABIN493209) Images back to top
|Alternative Name||PAK1 (PAK1 Antibody Abstract)|
|Background||A brain serine threonine protein kinase is a target for p21 ras related proteins Cdc42 and Rac1. p21-activated kinases (PAKs) bind to and are activated by Rho family GTPases such as Cdc42 and Rac. These GTPases play key roles in regulating cell polarity, stress responses, and cell cycle progression (1). Stiochiometeric analyses using purified Rac and PAK suggest that Rac binds to PAK with high affinity in a 1:1 ratio. The kinase complexes specifically with aGTP bound activated p21, inhibiting the p21 activity and leading to activation and auto-phosphorylation of p65pak (2). Three isoforms of p21-activated serine/threonine kinases, PAK68, PAK65 and PAK62, were found in both cytosolic & membrane fraction, & their contents were significantly increased during induced retinoic acid differentiation of human promyelocytic leukemia HL60 cell (3). The content of PAK65 or Rac is not evident in plasma membrane fraction of cells form un-differentiated cells (2). Constitutively activated Pak1 also suppress the activity of scavenger receptor class B, type 1 (SB-B1) in macrophage cell line RAW264.7, an action similar to LPS (4). Mutational studies with PAK1 also suggest that SR-B1 receptor down regulation involves binding to Rac and Cdc42. In mammalian cells, hyper osmotic shocks can induce apoptotic cell death. It is suggested that hyper osmotic shock can induce cleavage for PAK2 to produce a 36 kDa kinase, which is the result of DNA fragmentation and activation of ICE/CED-3 family cysteine proteases termed caspases-3.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK|
|Research Area||Cancer, Apoptosis/Necrosis, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 Antibody (ABIN493209) Images back to top
IMM and i. p pull-down assays: > 1: 100. Western blot: > 1: 250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 Antibody (ABIN493209) Images back to top
|Concentration||1.0 - 1.25 mg/mL|
|Buffer||Stabilization buffer with 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-PAK1 Antibody (ABIN493209)Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Nisimoto, Ogawa: "Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid." in: European journal of biochemistry / FEBS, Vol. 269, Issue 10, pp. 2622-9, 2002 (PubMed).
Hullinger, Panek, Xu et al.: "p21-activated kinase-1 (PAK1) inhibition of the human scavenger receptor class B, type I promoter in macrophages is independent of PAK1 kinase activity, but requires the GTPase-binding domain." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 46807-14, 2001 (PubMed).
Sells, Barratt, Caviston et al.: "Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18490-8, 1998 (PubMed).