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|Antigen||P21-Activated Kinase 2 (PAK2) Antibodies|
|Conjugate||This PAK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-PAK2 AntibodyTarget Details PAK2 Application Details Handling References for anti-PAK2 antibody (ABIN360318) Images
|Specificity||This antibody reacts to PAK2.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PAK2.|
Target Details PAK2Product Details anti-PAK2 Antibody Application Details Handling References for anti-PAK2 antibody (ABIN360318) Images back to top
|Alternative Name||PAK2 (PAK2 Antibody Abstract)|
|Background||PAK2, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. PAK2 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Expression is ubiquitous, with higher levels seen in skeletal muscle, ovary, thymus and spleen. PAK2 is autophosphorylated when activated by CDC42/p21. The protein structure contains 1 CRIB domain.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58|
|Gene ID||5062, 9606|
|Research Area||Cancer, Apoptosis/Necrosis, Tyrosine Kinases, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha|
Application DetailsProduct Details anti-PAK2 Antibody Target Details PAK2 Handling References for anti-PAK2 antibody (ABIN360318) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK2 Antibody Target Details PAK2 Application Details References for anti-PAK2 antibody (ABIN360318) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PAK2 antibody (ABIN360318)Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling Images back to top
Benner, Dennis, Masaracchia: "Activation of an S6/H4 kinase (PAK 65) from human placenta by intramolecular and intermolecular autophosphorylation." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 21121-8, 1995
Martin, Bollag, McCormick, Abo: "A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20." in: The EMBO journal, Vol. 14, Issue 9, pp. 1970-8, 1995
ImagesProduct Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling References for anti-PAK2 antibody (ABIN360318) back to top