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anti-Human PAK2 antibody for Enzyme Immunoassay

Recommended PAK2 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 2 (PAK2) Antibodies
  • 5330420P17Rik
  • A130002K10Rik
  • AI836325
  • cb422
  • D16Ertd269e
  • gamma-PAK
  • kinase
  • mKIAA4182
  • PAK-2
  • pak2
  • Pak3
  • pak65
  • PAK65
  • PAKgamma
  • pakgamma
  • PAKI
  • si:dz198m22.1
  • wu:fb71h01
  • zgc:91798
Epitope
N-Term
42
23
12
9
6
6
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
170
74
73
6
5
1
Host
Rabbit
155
15
Clonality
Polyclonal
Conjugate
This PAK2 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
150
97
73
20
14
5
2
2
2
1
Supplier
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Catalog No. ABIN360318
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087994966 ABIN500439 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.00087994966 ABIN500441 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 4

Top referenced anti-PAK2 antibody for Enzyme Immunoassay

Similar anti-PAK2 Antibodies

Application / Reactivity Human
ELISA 97 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen P21-Activated Kinase 2 (PAK2) Antibodies
Epitope N-Term
(42), (23), (12), (9), (6), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(170), (74), (73), (6), (5), (1)
Host Rabbit
(155), (15)
Clonality
Polyclonal
Conjugate This PAK2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(150), (97), (73), (20), (14), (5), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-PAK2 Antibody

Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN360318) Images
Specificity This antibody reacts to PAK2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PAK2.
Isotype Ig

Target Details PAK2

Product Details anti-PAK2 Antibody Application Details Handling References for anti-PAK2 Antibody (ABIN360318) Images back to top
Antigen
Alternative Name PAK2 (PAK2 Antibody Abstract)
Background PAK2, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. PAK2 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Expression is ubiquitous, with higher levels seen in skeletal muscle, ovary, thymus and spleen. PAK2 is autophosphorylated when activated by CDC42/p21. The protein structure contains 1 CRIB domain.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58
Gene ID 5062, 9606
UniProt Q13177
Research Area Cancer, Apoptosis/Necrosis, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK2 Antibody Target Details PAK2 Handling References for anti-PAK2 Antibody (ABIN360318) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details References for anti-PAK2 Antibody (ABIN360318) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PAK2 Antibody (ABIN360318)

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling Images back to top
Background publications

Benner, Dennis, Masaracchia: "Activation of an S6/H4 kinase (PAK 65) from human placenta by intramolecular and intermolecular autophosphorylation." in: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 21121-8, 1995

Martin, Bollag, McCormick, Abo: "A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20." in: The EMBO journal, Vol. 14, Issue 9, pp. 1970-8, 1995

Images

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN360318) back to top
Supplier Images
 image for anti-PAK2 antibody (P21-Activated Kinase 2) (N-Term) (ABIN360318) anti-P21-Activated Kinase 2 (PAK2) (N-Term) antibody
 image for anti-PAK2 antibody (P21-Activated Kinase 2) (N-Term) (ABIN360318) anti-P21-Activated Kinase 2 (PAK2) (N-Term) antibody (Image 2)