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anti-Human PAK2 antibody for Western Blotting

Recommended PAK2 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 2 (PAK2) Antibodies
  • 5330420P17Rik
  • A130002K10Rik
  • AI836325
  • cb422
  • D16Ertd269e
  • gamma-PAK
  • kinase
  • mKIAA4182
  • PAK-2
  • pak2
  • Pak3
  • pak65
  • PAK65
  • PAKgamma
  • pakgamma
  • PAKI
  • si:dz198m22.1
  • wu:fb71h01
  • zgc:91798
Epitope
C-Term
43
22
12
9
6
6
4
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
170
73
72
6
5
1
Host
Rabbit
155
15
Clonality
Polyclonal
Conjugate
This PAK2 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
150
97
73
20
14
5
2
2
2
1
Supplier
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Catalog No. ABIN500439
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN500441 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.12303223 ABIN1533101 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1533102 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN1533103 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12303223 ABIN392433 IHC (p) WB Rabbit Ig AA 192-222, N-Term Log in to see Polyclonal 2
0.12303223 ABIN969338 ICC IHC ELISA WB Mouse IgG1 Log in to see 3B5 2
0.12303223 ABIN223161 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1531457 IHC ELISA WB Rabbit IgG pSer20 Log in to see Polyclonal
0.12303223 ABIN1532070 ELISA WB Rabbit IgG pSer141 Log in to see Polyclonal
0.12303223 ABIN1108567 IF IHC (p) WB Mouse IgG1 Log in to see 3B5 3
0.12303223 ABIN499019 IHC (p) WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2707687 WB Rabbit N-Term, pSer20 Log in to see Polyclonal
0.12303223 ABIN256878 ICC FACS IF IHC (p) IHC WB Rabbit IgG N-Term Log in to see EP796Y
0.12303223 ABIN253074 IP WB Rabbit Log in to see Polyclonal
0.12303223 ABIN2476049 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.12303223 ABIN2498728 ELISA WB Rabbit IgG Ser192 Log in to see Polyclonal
0.12303223 ABIN2498729 ELISA WB Rabbit IgG Ser62 Log in to see Polyclonal
0.12303223 ABIN2498730 ELISA WB Rabbit IgG pSer141 Log in to see Polyclonal
0.12303223 ABIN2498727 ELISA WB Rabbit IgG Ser141 Log in to see Polyclonal
0.12303223 ABIN932933 IF IHC ELISA WB Mouse IgG1 Log in to see 3B5

Top referenced anti-PAK2 antibody for Western Blotting

Similar anti-PAK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 52 Antibodies 52 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 36 Antibodies 36 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 13 Antibodies
Western Blotting (WB) 63 Antibodies 64 Antibodies

General

Antigen P21-Activated Kinase 2 (PAK2) Antibodies
Epitope C-Term
(43), (22), (12), (9), (6), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(170), (73), (72), (6), (5), (1)
Host Rabbit
(155), (15)
Clonality
Polyclonal
Conjugate This PAK2 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(150), (97), (73), (20), (14), (5), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-PAK2 Antibody

Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN500439) Images
Specificity This antibody detects PAK2 at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen 14 amino acid peptide from near the carboxy terminus of human PAK2
Isotype IgG

Target Details PAK2

Product Details anti-PAK2 Antibody Application Details Handling References for anti-PAK2 Antibody (ABIN500439) Images back to top
Antigen
Alternative Name PAK2 (PAK2 Antibody Abstract)
Background The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58
Gene ID 5062
NCBI Accession NP_002568
UniProt Q13177
Research Area Cancer, Apoptosis/Necrosis, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK2 Antibody Target Details PAK2 Handling References for anti-PAK2 Antibody (ABIN500439) Images back to top
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details References for anti-PAK2 Antibody (ABIN500439) Images back to top
Concentration 1 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.

References for anti-PAK2 Antibody (ABIN500439)

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling Images back to top
Background publications

Ang, Patel, Yin, Sundaram, Fritchie, Zhao, Liu, Freemerman, Wilkerson, Walter, Weissler, Shockley, Couch, Zanation, Hackman, Chera, Harris, Miller, Thorne, Hayward, Funkhouser, Olshan, Shores, Makowski, Hayes: "High XRCC1 protein expression is associated with poorer survival in patients with head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6542-52, 2011

Vilas, Corvi, Plummer, Seime, Lambkin, Berthiaume: "Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6542-7, 2006

Jaffer, Chernoff: "p21-activated kinases: three more join the Pak." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 7, pp. 713-7, 2002

Rudel, Bokoch: "Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2." in: Science (New York, N.Y.), Vol. 276, Issue 5318, pp. 1571-4, 1997

Images

Product Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling References for anti-PAK2 Antibody (ABIN500439) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PAK2 antibody (P21-Activated Kinase 2) (C-Term) (ABIN500439) Immunohistochemistry of PAK2 in mouse spleen tissue with this product at 10 μg/ml.
Western Blotting (WB) image for anti-PAK2 antibody (P21-Activated Kinase 2) (C-Term) (ABIN500439) Western blot analysis of PAK2 in Jurkat lysate with this product at (A) 0.5 and (B) 1...