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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||165 Antibodies||82 Antibodies||78 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies||4 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||36 Antibodies||24 Antibodies||23 Antibodies|
|Immunohistochemistry (IHC)||76 Antibodies||38 Antibodies||38 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Antigen||P21-Activated Kinase 2 (PAK2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PAK2 AntibodyTarget Details PAK2 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PAKγ protein. (region surrounding Arg186)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details PAK2Product Details anti-PAK2 Antibody Application Details Handling Images back to top
|Alternative Name||PAK2 (PAK2 Antibody Abstract)|
|Background||Three isoforms of serine/threonine kinases, designated 7PAK p68, 8PAK p65 and 9PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The 7, 8, and 9 PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on 9 PAK, including Ser 19, Ser 141 Thr 402, phosphorylation of Ser 141 and Thr 402 is correlated with 9 PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4, which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP dependent manner with Rac 1 and Cdc 42, they do not interact with Rho.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58|
|Molecular Weight||approx. 58 kDa|
|Research Area||Cancer, Apoptosis/Necrosis, Tyrosine Kinases, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha|
Application DetailsProduct Details anti-PAK2 Antibody Target Details PAK2 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK2 Antibody Target Details PAK2 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-PAK2 Antibody Target Details PAK2 Application Details Handling back to top