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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||299 Antibodies||150 Antibodies||140 Antibodies||29 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||28 Antibodies||19 Antibodies||17 Antibodies||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||55 Antibodies||38 Antibodies||35 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||100 Antibodies||47 Antibodies|
|Antigen||Paxillin (PXN) Antibodies|
|Epitope||AA 305-559, C-Term Alternatives|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Paxillin antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Western Blotting (WB)
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Product Details anti-Paxillin AntibodyTarget Details Paxillin Application Details Handling Images
|Purpose||Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human,mouse,rat,chicken. No cross reactivity with other proteins.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human,mouse,rat,chicken.
Gene Name: paxillin
Protein Name: Paxillin
|Immunogen||C-terminal part of recombinant chicken paxillin(amino acids 305-559).|
Target Details PaxillinProduct Details anti-Paxillin Antibody Application Details Handling Images back to top
|Alternative Name||PXN (PXN Antibody Abstract)|
|Background||The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68- kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.|
|Gene ID||5829, 19303, 360820|
|Pathways||MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-Paxillin Antibody Target Details Paxillin Handling Images back to top
WB: Concentration: 1-2 μg/mL, Species: Human, mouse, rat, chicken
ICC: Concentration: 1 μg/mL, Species: Human, mouse, rat, chicken
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Images back to top
|Reconstitution||Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.|
|Buffer||Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling back to top