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anti-Chicken Paxillin antibody for Western Blotting

Recommended Paxillin Antibody (supplied by: Log in to see )

Antigen
Paxillin (PXN) Antibodies
  • AW108311
  • AW123232
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • Dmel\\CG31794
  • dPax
  • Dpax
  • DpaxA
  • DPaxillin
  • DPxn
  • DPxn37
  • LOC100220858
  • pax
  • Pax
  • PDLP
  • PXN
  • wu:fw71f12
Epitope
AA 1-557
60
50
25
24
19
17
13
13
9
8
7
7
7
6
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
366
163
149
24
5
4
3
2
2
1
1
1
1
1
Host
Mouse
333
47
2
Clonality
Monoclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
291
102
91
56
47
46
25
22
8
8
4
2
1
1
1
1
Supplier
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Catalog No. ABIN968042
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN968043 BI IP IHC WB Mouse IgG1 AA 1-557 Log in to see 177-Paxillin 5
0.12303223 ABIN968067 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 5
0.12303223 ABIN968066 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 4
0.12303223 ABIN967711 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.12303223 ABIN967712 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.12303223 ABIN3043666 ICC WB Mouse IgG1 AA 305-559, C-Term Log in to see PXC-10
0.0011806369 ABIN1108595 IF WB Mouse IgG1 C-Term, AA 305-559 Log in to see PAX-14 2
0.0011806369 ABIN1493425 ICC WB Mouse IgG1 C-Term Log in to see PAX-14
0.0011806369 ABIN597027 ICC WB Mouse IgG1 C-Term Log in to see
0.0011806369 ABIN1585566 ICC ELISA WB Mouse IgG1 C-Term, AA 305-559 Log in to see PAX-14
0.0011806369 ABIN2755718 WB Rabbit Center Log in to see Polyclonal
0.0011806369 ABIN201403 ICC IP IHC ELISA WB Mouse IgG1 Log in to see
0.0011806369 ABIN2331031 IC WB Mouse IgG1 AA 305-559 Log in to see 10K301
0.0011806369 ABIN2467642 IHC WB Rabbit IgG pTyr118 Log in to see Polyclonal
0.0011806369 ABIN2331028 IC IP IHC ELISA WB Mouse IgG1 Log in to see 2Q1106
0.0011806369 ABIN2498812 ICC IHC WB Mouse IgG1 AA 305-559, C-Term Log in to see PAX-14
0.0011806369 ABIN3032169 ICC WB Mouse IgG1 AA 305-559, C-Term Log in to see PAX-14
0.0011806369 ABIN2331026 IC WB Mouse IgG1 AA 305-559 Log in to see PAX-14
0.0011806369 ABIN3066297 WB Rabbit Center Log in to see Polyclonal
0.0011806369 ABIN1689540 ICC WB Mouse IgG1 Log in to see PAX-14

Top referenced anti-Paxillin antibody for Western Blotting

Similar anti-Paxillin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine)
Western Blotting (WB) 286 Antibodies 114 Antibodies 102 Antibodies 21 Antibodies 3 Antibodies 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 23 Antibodies 11 Antibodies 11 Antibodies 12 Antibodies 6 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies 24 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 102 Antibodies 43 Antibodies

General

Antigen Paxillin (PXN) Antibodies
Epitope AA 1-557
(60), (50), (25), (24), (19), (17), (13), (13), (9), (8), (7), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(366), (163), (149), (24), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(333), (47), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Paxillin antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
(291), (102), (91), (56), (47), (46), (25), (22), (8), (8), (4), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Paxillin Antibody

Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN968042) Images
Cross-Reactivity Human, Cow (Bovine), Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Chicken Paxillin aa. 1-557
Clone 177-Paxillin
Isotype IgG1

Target Details Paxillin

Product Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN968042) Images back to top
Antigen
Alternative Name Paxillin (PXN Antibody Abstract)
Background Paxillin, a focal adhesion protein, is a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. This is consisent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3-binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Although paxillin was initially detected in fibroblasts, its phosphorylation may be important during neurite extension during differentiation.
Molecular Weight 68 kDa
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN968042) Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN968042) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Paxillin Antibody (ABIN968042)

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
Product cited in:

Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, 2000

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, 1999

Leventhal, Feldman: "Tyrosine phosphorylation and enhanced expression of paxillin during neuronal differentiation in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 5957-60, 1996

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, 1995

Background publications

Turner, Glenney, Burridge: "Paxillin: a new vinculin-binding protein present in focal adhesions." in: The Journal of cell biology, Vol. 111, Issue 3, pp. 1059-68, 1990

Images

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN968042) back to top
Supplier Images
Western Blotting (WB) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968042) Western blot analysis of Paxillin on a human endothelial lysate. Lane 1: 1:1000, lane...
Immunofluorescence (IF) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968042) Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well...