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anti-Human Paxillin antibody for Enzyme Immunoassay

Recommended Paxillin Antibody (supplied by: Log in to see )

Antigen
Paxillin (PXN) Antibodies
  • AW108311
  • AW123232
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • Dmel\\CG31794
  • dPax
  • Dpax
  • DpaxA
  • DPaxillin
  • DPxn
  • DPxn37
  • LOC100220858
  • pax
  • Pax
  • PDLP
  • PXN
  • wu:fw71f12
Reactivity
Human, Mouse (Murine), Rat (Rattus)
374
169
155
26
7
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
340
48
2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
299
108
95
56
52
46
26
22
8
8
4
2
1
1
1
1
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Catalog No. ABIN271779
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854274 ABIN359002 EIA IHC (p) WB Rabbit Ig pTyr118 Log in to see Polyclonal

Similar anti-Paxillin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 38 Antibodies 40 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 11 Antibodies
Immunofluorescence (IF) 10 Antibodies 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies 45 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 108 Antibodies

General

Antigen Paxillin (PXN) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(374), (169), (155), (26), (7), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(340), (48), (2)
Clonality
Polyclonal
Conjugate This Paxillin antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(299), (108), (95), (56), (52), (46), (26), (22), (8), (8), (4), (2), (1), (1), (1), (1)
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Product Details anti-Paxillin Antibody

Target Details Paxillin Application Details Handling Images
Specificity This antibody detects endogenous levels of Paxillin protein. (region surrounding Gly112)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details Paxillin

Product Details anti-Paxillin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Paxillin / PXN (PXN Antibody Abstract)
Background Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.Synonyms: FLJ16691
Molecular Weight approx. 68 kDa
Gene ID 5829
NCBI Accession NP_001074324
UniProt P49023
Pathways MAPK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Paxillin Antibody Target Details Paxillin Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling back to top
Supplier Images
 image for anti-Paxillin antibody (PXN) (ABIN271779) anti-Paxillin (PXN) antibody
 image for anti-Paxillin antibody (PXN) (ABIN271779) anti-Paxillin (PXN) antibody (Image 2)