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anti-Human Paxillin antibody for Immunofluorescence

Recommended Paxillin Antibody (supplied by: Log in to see )

Antigen
Paxillin (PXN) Antibodies
  • AW108311
  • AW123232
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • Dmel\\CG31794
  • dPax
  • Dpax
  • DpaxA
  • DPaxillin
  • DPxn
  • DPxn37
  • LOC100220858
  • pax
  • Pax
  • PDLP
  • PXN
  • wu:fw71f12
Epitope
AA 1-557
61
50
25
24
19
17
13
13
10
9
7
7
7
6
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
374
169
155
25
6
4
3
3
2
2
1
1
1
1
1
Host
Mouse
341
47
2
Clonality
Monoclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
299
107
95
56
53
45
25
22
8
8
4
2
2
1
1
1
Supplier
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Catalog No. ABIN968067
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3218666 ABIN968066 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 4
0.3218666 ABIN967711 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.3218666 ABIN967712 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.3218666 ABIN189663 ICC IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y113
0.3218666 ABIN2707694 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.3218666 ABIN1870554 IF WB Rabbit IgG pTyr88 Log in to see Polyclonal
0.3218666 ABIN2498804 IF WB Rabbit IgG Tyr88 Log in to see Polyclonal
0.3218666 ABIN2498802 IF Rabbit IgG pTyr88 Log in to see Polyclonal
0.3218666 ABIN3020463 IF WB Rabbit pTyr88 Log in to see Polyclonal
0.3218666 ABIN349193 IF Rabbit IgG pTyr88 Log in to see Polyclonal
0.3218666 ABIN349194 IF Rabbit IgG pTyr88 Log in to see Polyclonal
0.3218666 ABIN349420 IF WB Rabbit IgG Log in to see Polyclonal
0.3218666 ABIN349419 IF WB Rabbit IgG Log in to see Polyclonal
0.0010826335 ABIN180343 IF IHC (p) IP WB Mouse IgG1 Log in to see 5H11 3
0.0010826335 ABIN1108595 IF WB Mouse IgG1 C-Term, AA 305-559 Log in to see PAX-14 2
0.0010826335 ABIN401592 IF WB Rabbit pTyr88 Log in to see Polyclonal 3
0.0010826335 ABIN401644 IF WB Rabbit Tyr88 Log in to see Polyclonal 4
0.0010826335 ABIN363106 IF WB Rabbit IgG AA 86-90 Log in to see Polyclonal 5
0.0010826335 ABIN362464 IF Rabbit IgG pTyr88 Log in to see Polyclonal 3
0.0010826335 ABIN1502620 IF IHC (p) IP WB Mouse kappa, IgG1 Log in to see 5H11

Top referenced anti-Paxillin antibody for Immunofluorescence

Similar anti-Paxillin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine)
Western Blotting (WB) 294 Antibodies 120 Antibodies 108 Antibodies 22 Antibodies 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 23 Antibodies 11 Antibodies 11 Antibodies 12 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies 29 Antibodies 29 Antibodies
Immunohistochemistry (IHC) 108 Antibodies 48 Antibodies 41 Antibodies 7 Antibodies

General

Antigen Paxillin (PXN) Antibodies
Epitope AA 1-557
(61), (50), (25), (24), (19), (17), (13), (13), (10), (9), (7), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(374), (169), (155), (25), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(341), (47), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Paxillin antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(299), (107), (95), (56), (53), (45), (25), (22), (8), (8), (4), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Paxillin Antibody

Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN968067) Images
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Chicken Paxillin aa. 1-557
Clone 165-Paxillin
Isotype IgG1

Target Details Paxillin

Product Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN968067) Images back to top
Antigen
Alternative Name Paxillin (PXN Antibody Abstract)
Background Paxillin, a focal adhesion protein, is a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. This is consisent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3 binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Paxillin was initially detected in fibroblasts, and its phosphorylation may be important during neurite extension during differentiation. This antibody is routinely tested by western blot analysis.
Molecular Weight 68 kDa
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN968067) Images back to top
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN968067) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-Paxillin Antibody (ABIN968067)

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
Product cited in:

Laukaitis, Webb, Donais, Horwitz: "Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1427-40, 2001

Müller, Jung, Wied, Welte, Jordan, Frick: "Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4553-67, 2001

Herreros, Rodríguez-Fernandez, Brown, Alonso-Lebrero, Cabañas, Sánchez-Madrid, Longo, Turner, Sánchez-Mateos: "Paxillin localizes to the lymphocyte microtubule organizing center and associates with the microtubule cytoskeleton." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26436-40, 2000

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, 1995

Turner, Glenney, Burridge: "Paxillin: a new vinculin-binding protein present in focal adhesions." in: The Journal of cell biology, Vol. 111, Issue 3, pp. 1059-68, 1990

Images

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN968067) back to top
Supplier Images
Western Blotting (WB) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968067) Western blot analysis of Paxillin of human endothelial cell lysate. Lane 1: 1:1000, l...
Immunofluorescence (IF) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968067) Immunofluorescent staining of WI38 cells with anti-Paxillin.
 image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968067) anti-Paxillin (PXN) (AA 1-557) antibody (Image 3)
Western Blotting (WB) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968067) anti-Paxillin (PXN) (AA 1-557) antibody (Image 4)