|Application / Reactivity||Human||Mouse (Murine)|
|Western Blotting (WB)||286 Antibodies||114 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||102 Antibodies||43 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||4 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||57 Antibodies||47 Antibodies|
|Immunofluorescence (IF)||47 Antibodies||10 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Antigen||Paxillin (PXN) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This Paxillin antibody is un-conjugated Alternatives|
Intracellular Flow Cytometry (ICFC)
|7 references available|
|Supplier||Log in to see|
Product Details anti-Paxillin AntibodyTarget Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN2666109) Images
|Purification||The antibody was purified by affinity chromatography.|
Target Details PaxillinProduct Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN2666109) Images back to top
|Alternative Name||Paxillin (PXN Antibody Abstract)|
|Background||Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as α, β, γ, approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-β1. The PAX227 monoclonal antibody recognizes human and mouse paxillin and has been shown to be useful for Western blotting.|
|Research Area||Cancer, Extracellular Matrix|
|Pathways||MAPK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN2666109) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN2666109) Images back to top
|Buffer||phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 50 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Upon receipt, store frozen at -20°C.|
References for anti-Paxillin Antibody (ABIN2666109)Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
Cai, Casimir, Schroer et al.: "In vivo role of focal adhesion kinase in regulating pancreatic β-cell mass and function through insulin signaling, actin dynamics, and granule trafficking." in: Diabetes, Vol. 61, Issue 7, pp. 1708-18, 2012 (PubMed).
Heinhuis, Koenders, van den Berg et al.: "Interleukin 32 (IL-32) contains a typical ?-helix bundle structure that resembles focal adhesion targeting region of focal adhesion kinase-1." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5733-43, 2012 (PubMed).
Yao, Dashner, van Golen et al.: "RhoC GTPase is required for PC-3 prostate cancer cell invasion but not motility." in: Oncogene, Vol. 25, Issue 16, pp. 2285-96, 2006 (PubMed).
Tang, Tan: "Role of Crk-associated substrate in the regulation of vascular smooth muscle contraction." in: Hypertension, Vol. 42, Issue 4, pp. 858-63, 2003 (PubMed).
Kirchner, Kam, Tzur et al.: "Live-cell monitoring of tyrosine phosphorylation in focal adhesions following microtubule disruption." in: Journal of cell science, Vol. 116, Issue Pt 6, pp. 975-86, 2003 (PubMed).
Mazaki, Hashimoto, Sabe: "Monocyte cells and cancer cells express novel paxillin isoforms with different binding properties to focal adhesion proteins." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7437-44, 1997 (PubMed).
Leventhal, Shelden, Kim et al.: "Tyrosine phosphorylation of paxillin and focal adhesion kinase during insulin-like growth factor-I-stimulated lamellipodial advance." in: The Journal of biological chemistry, Vol. 272, Issue 8, pp. 5214-8, 1997 (PubMed).
ImagesProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN2666109) back to top