anti-Mouse (Murine) Paxillin antibody for Western Blotting

Recommended Paxillin Antibody (supplied by: Log in to see )

Antigen
Paxillin (PXN) Antibodies
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • PXN
  • LOC100220858
  • AW108311
  • AW123232
  • Pax
  • wu:fw71f12
  • paxillin
  • Paxillin
  • paxillin-like
  • PXN
  • Pax
  • pxn
  • LOC100220858
  • Pxn
  • LOC100353305
Epitope
AA 1-557
67
53
29
21
16
15
14
14
13
10
9
8
7
6
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
398
209
197
31
12
10
10
8
5
5
4
3
1
1
1
1
1
1
Host
Mouse
361
45
2
Clonality
Monoclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
320
109
88
64
58
46
30
21
8
5
4
3
2
2
1
1
1
Options
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Catalog No. ABIN967711
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.942359 ABIN968043 BI IP IHC WB Mouse IgG1 AA 1-557 Log in to see 177-Paxillin 5
2.942359 ABIN968042 BI IP WB Mouse IgG1 AA 1-557 Log in to see 177-Paxillin 5
2.942359 ABIN447010 ICC IF IHC (p) IHC WB Rabbit N-Term Log in to see Polyclonal 1
2.942359 ABIN968066 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 4
2.942359 ABIN968067 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 5
2.942359 ABIN967712 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
2.942359 ABIN3043666 ICC WB Mouse IgG1 AA 305-559, C-Term Log in to see PXC-10 3
2.942359 ABIN3044187 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 456-472, C-Term Log in to see Polyclonal 3
2.942359 ABIN1531946 ELISA WB Rabbit IgG pTyr118 Log in to see Polyclonal
2.942359 ABIN1531947 ELISA WB Rabbit IgG pTyr31 Log in to see Polyclonal
2.942359 ABIN3182119 ELISA IHC WB Rabbit IgG pTyr31 Log in to see Polyclonal
2.942359 ABIN3186344 ELISA WB Rabbit IgG Thr508 Log in to see Polyclonal
2.942359 ABIN3186342 ELISA IHC WB Rabbit IgG Thr507 Log in to see Polyclonal
2.942359 ABIN3186343 ELISA IF IHC WB Rabbit IgG Thr507 Log in to see Polyclonal
2.942359 ABIN3186345 ELISA WB Rabbit IgG Thr505, Thr508 Log in to see Polyclonal
2.942359 ABIN3182118 ELISA WB Rabbit IgG pTyr118 Log in to see Polyclonal
2.942359 ABIN1532979 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.942359 ABIN1532978 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.942359 ABIN2706774 IP IHC WB Rabbit Center Log in to see Polyclonal
2.942359 ABIN2706775 WB Rabbit pTyr118 Log in to see Polyclonal

General

Antigen Paxillin (PXN) Antibodies
Epitope AA 1-557
(67), (53), (29), (21), (16), (15), (14), (14), (13), (10), (9), (8), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(398), (209), (197), (31), (12), (10), (10), (8), (5), (5), (4), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(361), (45), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Paxillin antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(320), (109), (88), (64), (58), (46), (30), (21), (8), (5), (4), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Paxillin Antibody

Target Details Paxillin Application Details Handling ProductDetails: References for anti-Paxillin antibody (ABIN967711) Images
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Chicken Paxillin aa. 1-557
Clone 349-Paxillin
Isotype IgG1

Target Details Paxillin

Product Details anti-Paxillin Antibody Application Details Handling ProductDetails: References for anti-Paxillin antibody (ABIN967711) Images back to top
Antigen
Alternative Name Paxillin (PXN Antibody Abstract)
Background A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68 kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation. This antibody is routinely tested by Western blot analysis.
Molecular Weight 68 kDa
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-Paxillin Antibody Target Details Paxillin Handling ProductDetails: References for anti-Paxillin antibody (ABIN967711) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details ProductDetails: References for anti-Paxillin antibody (ABIN967711) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

ProductDetails: References for anti-Paxillin antibody (ABIN967711)

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
Product cited in:

Even-Ram, Maoz, Pokroy, Reich, Katz, Gutwein, Altevogt, Bar-Shavit: "Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the alpha vbeta 5 integrin." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10952-62, 2001

Nikolopoulos, Turner: "Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion." in: The Journal of cell biology, Vol. 151, Issue 7, pp. 1435-48, 2001

van de Water, Houtepen, Huigsloot, Tijdens: "Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36183-93, 2001

Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, 1999

Background publications

Glenney, Zokas: "Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton." in: The Journal of cell biology, Vol. 108, Issue 6, pp. 2401-8, 1989

Images

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling ProductDetails: References for anti-Paxillin antibody (ABIN967711) back to top
Supplier Images
Western Blotting (WB) image for anti-Paxillin (PXN) (AA 1-557) antibody (ABIN967711) Western blot analysis of Paxillin on a A431 lysate. Lane 1: 1:10,000, lane 2: 1:20,00...
Immunofluorescence (IF) image for anti-Paxillin (PXN) (AA 1-557) antibody (ABIN967711) Immunofluoresence staining of human fibroblasts.