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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||286 Antibodies||114 Antibodies||102 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||11 Antibodies||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies||24 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||102 Antibodies||43 Antibodies||36 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||4 Antibodies||2 Antibodies|
|Antigen||Paxillin (PXN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Paxillin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Paxillin AntibodyTarget Details Paxillin Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-Paxillin protein, only when phosphorylated at Tyr31.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details PaxillinProduct Details anti-Paxillin Antibody Application Details Handling Images back to top
|Alternative Name||Paxillin / PXN (PXN Antibody Abstract)|
|Background||The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G protein-coupled receptors. In addition to G protein-coupled receptors, Angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the Src oncogene.Synonyms: FLJ16691|
|Molecular Weight||approx. 68 kDa|
|Pathways||MAPK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-Paxillin Antibody Target Details Paxillin Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling back to top