You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) Paxillin antibody for Immunoprecipitation

Recommended Paxillin Antibody (supplied by: Log in to see )

Antigen
Paxillin (PXN) Antibodies
  • AW108311
  • AW123232
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • Dmel\\CG31794
  • dPax
  • Dpax
  • DpaxA
  • DPaxillin
  • DPxn
  • DPxn37
  • LOC100220858
  • pax
  • Pax
  • PDLP
  • PXN
  • wu:fw71f12
Reactivity
Human, Rat (Rattus)
374
170
155
26
7
4
3
3
2
2
1
1
1
1
1
Host
Mouse
341
47
2
Clonality
Monoclonal
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
299
108
95
56
52
45
25
22
8
8
4
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN180343
$ 565.95
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089459505 ABIN968042 BI IP WB Mouse IgG1 AA 1-557 Log in to see 177-Paxillin 5
0.00089459505 ABIN968043 BI IP IHC WB Mouse IgG1 AA 1-557 Log in to see 177-Paxillin 5
0.00089459505 ABIN968067 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 5
0.00089459505 ABIN968066 IF IP IHC WB Mouse IgG1 AA 1-557 Log in to see 165-Paxillin 4
0.00089459505 ABIN967711 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.00089459505 ABIN967712 IHC (f) IF IP WB Mouse IgG1 AA 1-557 Log in to see 349-Paxillin 5
0.00089459505 ABIN189663 ICC IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y113
0.00089459505 ABIN2706774 IP IHC WB Rabbit Center Log in to see Polyclonal
0.00089459505 ABIN1690682 IP Sepharose Mouse IgG1 Log in to see PAX-14
0.00089459505 ABIN1690335 IP Magnetic Particles Mouse IgG1 Log in to see PAX-14

Top referenced anti-Paxillin antibody for Immunoprecipitation

Similar anti-Paxillin Antibodies

Application / Reactivity Rat (Rattus) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 38 Antibodies 89 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 108 Antibodies
Immunochromatography (IC) 5 Antibodies

General

Antigen Paxillin (PXN) Antibodies
Reactivity Human, Rat (Rattus)
(374), (170), (155), (26), (7), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(341), (47), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Paxillin antibody is un-conjugated
(7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(299), (108), (95), (56), (52), (45), (25), (22), (8), (8), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-Paxillin Antibody

Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN180343) Images
Specificity Highly specific for Paxilin.
Cellular Localization: cell membrane, cytoplasmic.
Characteristics Synonyms: FLJ16691
Purification Affinity Chromatography on Protein G.
Immunogen Purified recombinant full-length human paxillin protein.
Clone 5H11
Isotype IgG1

Target Details Paxillin

Product Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN180343) Images back to top
Antigen
Alternative Name Paxillin / PXN (PXN Antibody Abstract)
Background Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691
Gene ID 5829
UniProt P49023
Pathways MAPK Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN180343) Images back to top
Application Notes Immunofluorescence. Immunoprecipitation (Native): use Protein G and Ab at 2 g/mgprotein lysate. Western Blotting (1-2 μg/mL for 2hrs at RT). Immunohistology(Formalin/paraffin, 1-2 μg/mL for 30 min at RT, Staining of formalin-fixed tissues REQUIRESboiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 mins followed by cooling at RT for 20 mins, Recommended positive control: HeLa Cells, Colon Carcinoma).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN180343) Images back to top
Concentration 0.2 mg/mL
Buffer 10 mM PBS with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-Paxillin Antibody (ABIN180343)

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
Background publications

de Curtis, Malanchini: "Integrin-mediated tyrosine phosphorylation and redistribution of paxillin during neuronal adhesion." in: Experimental cell research, Vol. 230, Issue 2, pp. 233-43, 1997

Leventhal, Shelden, Kim, Feldman: "Tyrosine phosphorylation of paxillin and focal adhesion kinase during insulin-like growth factor-I-stimulated lamellipodial advance." in: The Journal of biological chemistry, Vol. 272, Issue 8, pp. 5214-8, 1997

Hanks, Polte: "Signaling through focal adhesion kinase." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 19, Issue 2, pp. 137-45, 1997

Images

Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN180343) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Paxillin antibody (PXN) (ABIN180343) anti-Paxillin (PXN) antibody