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|Application / Reactivity||Rat (Rattus)||Human|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||44 Antibodies||83 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||14 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||53 Antibodies|
|Immunohistochemistry (IHC)||40 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||38 Antibodies|
|Immunoprecipitation (IP)||19 Antibodies|
|Western Blotting (WB)||136 Antibodies|
|Antigen||Paxillin (PXN) Antibodies|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Paxillin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Paxillin AntibodyTarget Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN180343) Images
Highly specific for Paxilin.
Cellular Localization: cell membrane, cytoplasmic.
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Purified recombinant full-length human paxillin protein.|
Target Details PaxillinProduct Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN180343) Images back to top
|Alternative Name||Paxillin / PXN (PXN Antibody Abstract)|
|Background||Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691|
|Pathways||MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN180343) Images back to top
Immunofluorescence. Immunoprecipitation (Native): use Protein G and Ab at 2 g/mgprotein lysate. Western Blotting (1-2 μg/mL for 2hrs at RT). Immunohistology(Formalin/paraffin, 1-2 μg/mL for 30 min at RT, Staining of formalin-fixed tissues REQUIRESboiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 mins followed by cooling at RT for 20 mins, Recommended positive control: HeLa Cells, Colon Carcinoma).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN180343) Images back to top
|Buffer||10 mM PBS with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Paxillin Antibody (ABIN180343)Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
de Curtis, Malanchini: "Integrin-mediated tyrosine phosphorylation and redistribution of paxillin during neuronal adhesion." in: Experimental cell research, Vol. 230, Issue 2, pp. 233-43, 1997
Leventhal, Shelden, Kim, Feldman: "Tyrosine phosphorylation of paxillin and focal adhesion kinase during insulin-like growth factor-I-stimulated lamellipodial advance." in: The Journal of biological chemistry, Vol. 272, Issue 8, pp. 5214-8, 1997
Hanks, Polte: "Signaling through focal adhesion kinase." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 19, Issue 2, pp. 137-45, 1997
ImagesProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN180343) back to top