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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||2 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies|
|Gel Shift (GS)||1 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||35 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||32 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||187 Antibodies|
|Antigen||Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies|
|Conjugate||This PPARG antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PPARG AntibodyTarget Details PPARG Application Details Handling References for anti-PPARG Antibody (ABIN117930) Images
|Sequence||Peptide sequence: M-G-E-T-L-G-D-S-P-I-D-P-E-S-D-S|
|Specificity||This antibody is directed against Human PPAR gamma isoform 2 protein. A BLAST analysis was used to suggest reactivity with this protein from Human and Macaque based on 100 % homology for the immunogen sequence. Cross reactivity with PPARg2 protein from Dog and Swine is likely due to 94 % homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from Mouse. Mouse PPARg2 shows 87 % homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.|
|Purification||Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.|
|Immunogen||This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2. PPAR gamma 2 is a 55 kDa protein.|
Target Details PPARGProduct Details anti-PPARG Antibody Application Details Handling References for anti-PPARG Antibody (ABIN117930) Images back to top
|Alternative Name||PPAR-gamma (PPARG Antibody Abstract)|
|Background||Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma|
|Pathways||MAPK Signaling, Nuclear Receptor Transcription Pathway|
Application DetailsProduct Details anti-PPARG Antibody Target Details PPARG Handling References for anti-PPARG Antibody (ABIN117930) Images back to top
|Application Notes||This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 40,000) and byWestern blot (1: 1,000-1: 3,000). Specific conditions for reactivity should be optimized bythe end user. Expect a single band approximately 55 kDa in size corresponding to PPARγ2|
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPARG Antibody Target Details PPARG Application Details References for anti-PPARG Antibody (ABIN117930) Images back to top
|Concentration||1.0 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.|
References for anti-PPARG Antibody (ABIN117930)Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling Images back to top
De Pascale, Bassi, Patrone et al.: "Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes." in: Archives of biochemistry and biophysics, Vol. 447, Issue 2, pp. 97-106, 2006 (PubMed).
Sastre, Dewachter, Rossner et al.: "Nonsteroidal anti-inflammatory drugs repress beta-secretase gene promoter activity by the activation of PPARgamma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 2, pp. 443-8, 2006 (PubMed).
Lodillinsky, Umerez, Jasnis et al.: "Bacillus Calmette-Guérin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells." in: International journal of molecular medicine, Vol. 17, Issue 2, pp. 269-73, 2006 (PubMed).
Schild, Sonnenberg-Hirche, Schaiff et al.: "The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts." in: Placenta, Vol. 27, Issue 2-3, pp. 191-9, 2005 (PubMed).
Pandhare, Cooper, Phang: "Proline oxidase, a proapoptotic gene, is induced by troglitazone: evidence for both peroxisome proliferator-activated receptor gamma-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 281, Issue 4, pp. 2044-52, 2006 (PubMed).
ImagesProduct Details anti-PPARG Antibody Target Details PPARG Application Details Handling References for anti-PPARG Antibody (ABIN117930) back to top