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anti-Human PPARG antibody for Enzyme Immunoassay

Recommended PPARG Antibody (supplied by: Log in to see )

Antigen
Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
  • CIMT1
  • GLM1
  • NR1C3
  • Nr1c3
  • PPAR-GAMMA
  • PPAR-gamma
  • PPAR-gamma2
  • PPARG
  • PPARG1
  • PPARg2
  • PPARG2
  • PPARGAMMA
  • PPARgamma
  • PPAR gamma
  • PPARgamma2
  • xPPAR-gamma
Epitope
N-Term
50
23
17
15
13
13
13
11
11
9
8
7
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
258
113
91
18
14
8
8
6
5
4
4
4
3
2
1
1
1
1
1
Host
Rabbit
249
28
5
5
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
18
15
9
3
3
3
3
3
3
3
3
3
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
214
122
51
44
33
17
15
13
9
8
6
6
2
2
1
1
Supplier
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Catalog No. ABIN117930
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN318834 EIA Rabbit IgG Isoform 1, AA 255-268 Log in to see Polyclonal 1
0.0008867729 ABIN302768 EIA IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0008867729 ABIN117929 EIA WB Rabbit Log in to see Polyclonal 3
0.0008867729 ABIN614415 EIA IHC (p) WB Mouse IgG1 AA 170-270 Log in to see 3A4A9-1E6A1
0.0008867729 ABIN303335 EIA IHC (p) WB Rabbit AA 255-268, Isoform 1, N-Term Log in to see Polyclonal
0.0008867729 ABIN1108704 EIA WB Mouse IgG1 Log in to see 3A4A9 3
0.0008867729 ABIN2729420 EIA Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN613291 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.0008867729 ABIN613765 EIA RIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-PPARG antibody for Enzyme Immunoassay

Similar anti-PPARG Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
ELISA 104 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 197 Antibodies

General

Antigen Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
Epitope N-Term
(50), (23), (17), (15), (13), (13), (13), (11), (11), (9), (8), (7), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(258), (113), (91), (18), (14), (8), (8), (6), (5), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(249), (28), (5), (5)
Clonality
Polyclonal
Conjugate This PPARG antibody is un-conjugated
(18), (15), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(214), (122), (51), (44), (33), (17), (15), (13), (9), (8), (6), (6), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-PPARG Antibody

Target Details PPARG Application Details Handling References for anti-PPARG Antibody (ABIN117930) Images
Sequence Peptide sequence: M-G-E-T-L-G-D-S-P-I-D-P-E-S-D-S
Specificity This antibody is directed against Human PPAR gamma isoform 2 protein. A BLAST analysis was used to suggest reactivity with this protein from Human and Macaque based on 100 % homology for the immunogen sequence. Cross reactivity with PPARg2 protein from Dog and Swine is likely due to 94 % homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from Mouse. Mouse PPARg2 shows 87 % homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2. PPAR gamma 2 is a 55 kDa protein.

Target Details PPARG

Product Details anti-PPARG Antibody Application Details Handling References for anti-PPARG Antibody (ABIN117930) Images back to top
Antigen
Alternative Name PPAR-gamma (PPARG Antibody Abstract)
Background Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma
Gene ID 5468
NCBI Accession NP_005028
UniProt P37231
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway

Application Details

Product Details anti-PPARG Antibody Target Details PPARG Handling References for anti-PPARG Antibody (ABIN117930) Images back to top
Application Notes This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 40,000) and byWestern blot (1: 1,000-1: 3,000). Specific conditions for reactivity should be optimized bythe end user. Expect a single band approximately 55 kDa in size corresponding to PPARγ2
Restrictions For Research Use only

Handling

Product Details anti-PPARG Antibody Target Details PPARG Application Details References for anti-PPARG Antibody (ABIN117930) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

References for anti-PPARG Antibody (ABIN117930)

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling Images back to top
Background publications

Lodillinsky, Umerez, Jasnis, Casabé, Sandes, Eiján: "Bacillus Calmette-Guérin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells." in: International journal of molecular medicine, Vol. 17, Issue 2, pp. 269-73, 2006

De Pascale, Bassi, Patrone, Villacorta, Azzi, Zingg: "Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes." in: Archives of biochemistry and biophysics, Vol. 447, Issue 2, pp. 97-106, 2006

Pandhare, Cooper, Phang: "Proline oxidase, a proapoptotic gene, is induced by troglitazone: evidence for both peroxisome proliferator-activated receptor gamma-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 281, Issue 4, pp. 2044-52, 2006

Sastre, Dewachter, Rossner, Bogdanovic, Rosen, Borghgraef, Evert, Dumitrescu-Ozimek, Thal, Landreth, Walter, Klockgether, van Leuven, Heneka: "Nonsteroidal anti-inflammatory drugs repress beta-secretase gene promoter activity by the activation of PPARgamma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 2, pp. 443-8, 2006

Schild, Sonnenberg-Hirche, Schaiff, Bildirici, Nelson, Sadovsky: "The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts." in: Placenta, Vol. 27, Issue 2-3, pp. 191-9, 2005

Images

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling References for anti-PPARG Antibody (ABIN117930) back to top
Supplier Images
Western Blotting (WB) image for anti-PPARG antibody (Peroxisome Proliferator-Activated Receptor gamma) (N-Term) (ABIN117930) Figure 1. Western blot using affinity purified anti-PPAR³2 antibody shows detection o...