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anti-Dog (Canine) RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pSer301
72
69
46
37
36
35
28
28
28
26
22
22
21
20
19
18
16
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis
782
400
378
23
22
16
14
13
7
7
7
5
3
2
2
1
1
1
1
1
Host
Rabbit
748
41
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
467
343
202
172
156
90
49
32
19
18
17
7
2
2
2
1
Supplier
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Catalog No. ABIN372711
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372712 WB Rabbit IgG pSer642 Log in to see Polyclonal 2
1 ABIN318805 WB Rabbit IgG pSer642 Log in to see Polyclonal
1 ABIN295906 IP WB Sheep IgG AA 657-668 Log in to see Polyclonal
1 ABIN602224 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
1 ABIN2476311 WB Rabbit IgG pSer642 Log in to see Polyclonal 2
1 ABIN602225 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN597445 WB Rabbit IgG Log in to see Polyclonal
1 ABIN209208 WB Rat IgG1 AA 334-347, pSer338 Log in to see
1 ABIN303955 WB Rabbit pSer642 Log in to see Polyclonal
1 ABIN1582169 ELISA WB Rabbit pSer301 Log in to see Polyclonal
1 ABIN1582164 ELISA WB Rabbit pSer642 Log in to see Polyclonal
1 ABIN1929029 IP WB Rabbit IgG AA 598-648 Log in to see Polyclonal
1 ABIN2959895 WB Rabbit IgG pSer642 Log in to see Polyclonal
1 ABIN2959896 WB Rabbit IgG pSer301 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Non Human Primates Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 6 Antibodies 6 Antibodies 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 442 Antibodies 248 Antibodies 227 Antibodies 23 Antibodies 15 Antibodies 22 Antibodies 2 Antibodies 8 Antibodies
RNA Interference (RNAi) 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer301
(72), (69), (46), (37), (36), (35), (28), (28), (28), (26), (22), (22), (21), (20), (19), (18), (16), (15), (15), (15), (15), (15), (14), (14), (10), (8), (8), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Xenopus laevis
(782), (400), (378), (23), (22), (16), (14), (13), (7), (7), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(748), (41), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(467), (343), (202), (172), (156), (90), (49), (32), (19), (18), (17), (7), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN372711) Images
Specificity This antibody recognizes the ~74k Raf-1 protein phosphorylated at Ser301. Immunolabeling of the Raf-1 protein band is blocked by l-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Human, Bovine, Canine, Mouse, Xenopus and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf1.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN372711) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 24703
NCBI Accession NP_036771
UniProt P11345
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN372711) Images back to top
Application Notes Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN372711) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-RAF1 Antibody (ABIN372711)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Chong, Vikis, Guan: "Mechanisms of regulating the Raf kinase family." in: Cellular signalling, Vol. 15, Issue 5, pp. 463-9, 2003

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN372711) back to top
Supplier Images
 image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer301) (ABIN372711) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer301) antibody