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anti-Human RAF1 antibody for Immunohistochemistry

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pThr269
75
64
56
38
38
36
29
29
28
28
24
23
21
21
20
19
17
16
15
15
15
15
15
15
14
12
9
8
8
6
6
6
6
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
816
361
345
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
769
41
14
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
12
12
Application
Immunohistochemistry (IHC), ELISA
467
354
208
182
156
76
42
32
18
17
12
2
2
2
1
Options
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Catalog No. ABIN1532076
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.600743 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
8.600743 ABIN1532077 IHC ELISA Rabbit IgG pSer289 Log in to see Polyclonal 1
8.600743 ABIN1533109 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
8.600743 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
8.600743 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
8.600743 ABIN969560 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
8.600743 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
8.600743 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
8.600743 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
8.600743 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
8.600743 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
8.600743 ABIN2469686 IHC WB Rabbit IgG Ser259 Log in to see Polyclonal
8.600743 ABIN1533110 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.600743 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.600743 ABIN1532444 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
8.600743 ABIN2957891 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
8.600743 ABIN3181567 IHC WB Rabbit IgG Log in to see Polyclonal
8.600743 ABIN2500173 IHC ELISA Rabbit IgG pThr269 Log in to see Polyclonal
8.600743 ABIN2500183 IHC ELISA WB Rabbit IgG Ser189 Log in to see Polyclonal
8.600743 ABIN2500189 IF IHC ELISA WB Rabbit IgG Ser621 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunohistochemistry

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 452 Antibodies 208 Antibodies 194 Antibodies
RNA Interference (RNAi) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 17 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 76 Antibodies 53 Antibodies 49 Antibodies
Immunohistochemistry (IHC) 207 Antibodies 87 Antibodies 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pThr269
(75), (64), (56), (38), (38), (36), (29), (29), (28), (28), (24), (23), (21), (21), (20), (19), (17), (16), (15), (15), (15), (15), (15), (15), (14), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(816), (361), (345), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(769), (41), (14), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunohistochemistry (IHC), ELISA
(467), (354), (208), (182), (156), (76), (42), (32), (18), (17), (12), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1532076) Images
Specificity C-RAF (Phospho-Thr269) Antibody detects endogenous levels of C- RAF only when phosphorylated at Thr269.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Thr269.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN1532076) Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background Molecular weight of target protein: 73 kDa
Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto- oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Molecular Weight 73 kDa
Gene ID 5894
OMIM 164760
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN1532076) Images back to top
Application Notes IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.159130 (NCBI Gene Symbol: RAF1)

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN1532076) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN1532076)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1532076) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pThr269) (ABIN1532076) Immunohistochemistry analysis of paraffin-embedded human brain, using C-RAF (Phospho-...