anti-Human RAF1 antibody for Immunohistochemistry

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pSer259
73
71
47
40
39
37
28
28
28
27
22
22
22
20
19
18
17
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
808
410
385
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rabbit
769
46
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
489
347
201
172
156
104
50
34
19
18
17
7
2
2
2
1
Options
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Catalog No. ABIN1531958
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.645801 ABIN1532076 IHC ELISA Rabbit IgG pThr269 Log in to see Polyclonal 1
9.645801 ABIN1532077 IHC ELISA Rabbit IgG pSer289 Log in to see Polyclonal 1
9.645801 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.645801 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.645801 ABIN1533109 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
9.645801 ABIN969560 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
9.645801 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
9.645801 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
9.645801 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
9.645801 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
9.645801 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
9.645801 ABIN2469686 IHC WB Rabbit IgG Ser259 Log in to see Polyclonal
9.645801 ABIN1533110 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.645801 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.645801 ABIN1532444 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.645801 ABIN2957891 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
9.645801 ABIN3181567 IHC WB Rabbit IgG Log in to see Polyclonal
9.645801 ABIN4349225 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 4G4
9.645801 ABIN2852532 IHC WB Rabbit Log in to see Polyclonal
9.645801 ABIN1846392 FACS IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-RAF1 antibody for Immunohistochemistry

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 90 Antibodies 96 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 143 Antibodies
Immunohistochemistry (IHC) 76 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 71 Antibodies
Immunoprecipitation (IP) 11 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 234 Antibodies
Flow Cytometry (FACS) 11 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer259
(73), (71), (47), (40), (39), (37), (28), (28), (28), (27), (22), (22), (22), (20), (19), (18), (17), (15), (15), (15), (15), (15), (14), (14), (10), (8), (8), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(808), (410), (385), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(769), (46), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(489), (347), (201), (172), (156), (104), (50), (34), (19), (18), (17), (7), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN1531958) Images
Specificity C-RAF (Phospho-Ser259) Antibody detects endogenous levels of C- RAF only when phosphorylated at Ser259.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser259.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN1531958) Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background Molecular weight of target protein: 73 kDa
Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto- oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Molecular Weight 73 kDa
Gene ID 5895
OMIM 164760
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling ProductDetails: References for anti-RAF1 antibody (ABIN1531958) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.159131 (NCBI Gene Symbol: RAF1)

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details ProductDetails: References for anti-RAF1 antibody (ABIN1531958) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-RAF1 antibody (ABIN1531958)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

King, Sun, Diaz, Barnard, Miao, Bagrodia, Marshall: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN1531958) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259) (ABIN1531958) Western blot analysis of extracts from K562 cells, using C-RAF (Phospho-Ser259) Antib...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259) (ABIN1531958) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C-RA...