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|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies|
|Conjugate||This RAF1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN119063) Images
|Specificity||This antibody recognises activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.|
|Purification||Affinity chromatography on Protein G|
|Immunogen||Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde.|
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 antibody (ABIN119063) Images back to top
|Alternative Name||RAF1 (RAF1 Antibody Abstract)|
|Background||Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf|
|Research Area||Signaling, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling|
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 antibody (ABIN119063) Images back to top
Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 antibody (ABIN119063) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-RAF1 antibody (ABIN119063)Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Mason, Springer, Cooper, Superti-Furga, Marshall, Marais: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999
ImagesProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN119063) back to top