anti-Human RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pSer338
79
74
47
47
46
43
41
34
34
28
27
23
21
19
18
18
15
15
15
15
15
15
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
834
412
387
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rat
780
63
11
6
2
Clonality
Monoclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
512
365
221
172
156
103
52
49
20
18
18
7
2
2
2
1
Supplier
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Catalog No. ABIN119063
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.5717 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
11.5717 ABIN214062 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
11.5717 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN499021 IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN498802 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
11.5717 ABIN4349224 IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN4349220 IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN4349222 IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.5717 ABIN4349226 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN733208 IF (p) IHC (p) WB Rabbit IgG AA 25-60 Log in to see Polyclonal 1
1 ABIN360145 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 2
1 ABIN712706 IF (p) IHC (p) WB Rabbit IgG AA 325-375, pTyr341 Log in to see Polyclonal 1
1 ABIN392261 FACS IF IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
1 ABIN196618 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal 5
1 ABIN197102 IHC (p) WB Rabbit Ser259 Log in to see Polyclonal 6
1 ABIN319325 IHC (p) WB Rabbit Ser338 Log in to see Polyclonal 3
1 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
1 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer338
(79), (74), (47), (47), (46), (43), (41), (34), (34), (28), (27), (23), (21), (19), (18), (18), (15), (15), (15), (15), (15), (15), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(834), (412), (387), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Rat
(780), (63), (11), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(512), (365), (221), (172), (156), (103), (52), (49), (20), (18), (18), (7), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN119063) Images
Specificity This antibody recognises activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Purification Affinity chromatography on Protein G
Immunogen Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde.
Clone P5338 (DPR-2-2A-3A)
Isotype IgG1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 antibody (ABIN119063) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 antibody (ABIN119063) Images back to top
Application Notes Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 antibody (ABIN119063) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RAF1 antibody (ABIN119063)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Mason, Springer, Cooper, Superti-Furga, Marshall, Marais: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 antibody (ABIN119063) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibody (ABIN119063) Formalin-fixed, paraffin-embedded human tonsil stained with SM1489 (Note nuclear stai...