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anti-Human RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
pSer338
73
62
56
39
36
34
27
26
26
26
23
23
20
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
787
337
319
18
18
10
5
4
4
2
2
2
1
1
Host
Rat
739
39
15
7
2
Clonality
Monoclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
457
352
205
181
132
78
39
30
18
16
11
2
2
2
Supplier
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Catalog No. ABIN119063
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34253615 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
0.34253615 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
0.34253615 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
0.34253615 ABIN499021 IHC (p) WB Rabbit Log in to see Polyclonal
0.34253615 ABIN214062 IHC (p) WB Rabbit IgG Ser259 Log in to see Polyclonal
0.34253615 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.34253615 ABIN498802 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
0.0008985059 ABIN733208 IF (p) IHC (p) WB Rabbit IgG AA 25-60 Log in to see Polyclonal 1
0.0008985059 ABIN360145 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 2
0.0008985059 ABIN392261 FACS IF IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.0008985059 ABIN197102 IHC (p) WB Rabbit Ser259 Log in to see Polyclonal 6
0.0008985059 ABIN196618 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal 5
0.0008985059 ABIN712706 IF (p) IHC (p) WB Rabbit IgG AA 325-375, pTyr341 Log in to see Polyclonal 1
0.0008985059 ABIN271937 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008985059 ABIN319325 IHC (p) WB Rabbit Ser338 Log in to see Polyclonal 3
0.0008985059 ABIN271798 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008985059 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
0.0008985059 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
0.0008985059 ABIN302942 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
0.0008985059 ABIN318109 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-RAF1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 181 Antibodies
ELISA 350 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies
Immunohistochemistry (IHC) 204 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 448 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer338
(73), (62), (56), (39), (36), (34), (27), (26), (26), (26), (23), (23), (20), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(787), (337), (319), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rat
(739), (39), (15), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(457), (352), (205), (181), (132), (78), (39), (30), (18), (16), (11), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN119063) Images
Specificity This antibody recognises activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Purification Affinity chromatography on Protein G
Immunogen Synthetic peptide GQRDpSSYpYWEIEAS coupled to glutaraldehyde.
Clone P5338 (DPR-2-2A-3A)
Isotype IgG1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN119063) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN119063) Images back to top
Application Notes Western Blot: 1/100 - 1/1000, This antibody may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN119063) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RAF1 Antibody (ABIN119063)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Mason, Springer, Cooper, Superti-Furga, Marshall, Marais: "Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation." in: The EMBO journal, Vol. 18, Issue 8, pp. 2137-48, 1999

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN119063) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer338) (ABIN119063) Formalin-fixed, paraffin-embedded human tonsil stained with SM1489 (Note nuclear stai...