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anti-Human RAF1 antibody for Immunoprecipitation

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
C-Term
74
61
56
39
36
34
27
26
26
26
23
22
20
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
780
332
314
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
732
38
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
450
351
199
181
132
75
39
29
18
15
11
2
2
2
Supplier
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Catalog No. ABIN222938
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089547504 ABIN127014 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.00089547504 ABIN233707 IP WB Rabbit IgG Internal Region Log in to see Polyclonal
0.00089547504 ABIN295906 IP WB Sheep IgG C-Term Log in to see Polyclonal
0.00089547504 ABIN241660 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.00089547504 ABIN233710 IP WB Rabbit IgG pSer259 Log in to see Polyclonal
0.00089547504 ABIN209207 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089547504 ABIN1003059 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089547504 ABIN233706 IP WB Rabbit Pro302 Log in to see Polyclonal
0.00089547504 ABIN229636 IF IP IHC WB Rabbit IgG Log in to see
0.00089547504 ABIN1929029 IP WB Rabbit IgG AA 598-648 Log in to see Polyclonal
0.00089547504 ABIN2347789 IP WB Sheep IgG C-Term, AA 637-648 Log in to see Polyclonal
0.00089547504 ABIN2347790 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089547504 ABIN2347795 IP ELISA WB Rabbit IgG Middle Region Log in to see Polyclonal
0.00089547504 ABIN2347666 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089547504 ABIN2150273 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunoprecipitation

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 85 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 20 Antibodies 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 121 Antibodies 121 Antibodies
Immunohistochemistry (IHC) 73 Antibodies 83 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 50 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 187 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope C-Term
(74), (61), (56), (39), (36), (34), (27), (26), (26), (26), (23), (22), (20), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(780), (332), (314), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(732), (38), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(450), (351), (199), (181), (132), (75), (39), (29), (18), (15), (11), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images
Blocking Peptide Blocking peptide for this product available: ABIN412894
Specificity The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots.
Cross-Reactivity (Details) It is non cross-reactive with Raf-A and Raf-B.
Purification Affinity purified
Immunogen Synthetic peptide mapping to the C-terminus of human Raf-1
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Antigen
Alternative Name Raf1 (RAF1 Antibody Abstract)
Background Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Synonyms: RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
Gene ID 5894
UniProt P04049
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Application Notes Western blot analysis (1-4 μg/mL), Immunoprecipitation (15-25 μg/mL) and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN222938) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN222938)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Ito, Nagayasu, Okumura-Noji, Lu, Nishida, Miura, Asai, Kheirollah, Nakaya, Yokoyama: "Mechanism for FGF-1 to regulate biogenesis of apoE-HDL in astrocytes." in: Journal of lipid research, Vol. 48, Issue 9, pp. 2020-7, 2007

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (C-Term) (ABIN222938) Western blot With Raf-l antibody, using rat kidney lysate (60 µg).