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anti-Human RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
AA 1-30, N-Term
75
71
48
39
37
36
29
29
28
28
23
23
22
21
20
19
17
15
15
15
15
15
14
14
11
9
9
8
8
6
6
6
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
819
426
402
26
25
17
16
13
9
7
7
5
4
3
2
2
1
1
1
1
Host
Rabbit
783
41
14
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
493
365
216
182
156
89
51
33
19
18
17
7
2
2
2
1
Options
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Catalog No. ABIN392261
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN222938 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 1
2.9590726 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
2.9590726 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
2.9590726 ABIN781705 EIA WB Chicken N-Term Log in to see Polyclonal 2
2.9590726 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.9590726 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.9590726 ABIN969560 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
2.9590726 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
2.9590726 ABIN389747 DB WB Rabbit Ig pSer338 Log in to see Polyclonal
2.9590726 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
2.9590726 ABIN126882 WB Mouse IgG1 Log in to see PBB-1
2.9590726 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
2.9590726 ABIN2855608 WB Rabbit IgG Center Log in to see Polyclonal
2.9590726 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
2.9590726 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
2.9590726 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
2.9590726 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
2.9590726 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
2.9590726 ABIN1870559 IF WB Rabbit IgG pSer621 Log in to see Polyclonal
2.9590726 ABIN3186668 ELISA WB Rabbit IgG Thr560 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 182 Antibodies
ELISA 364 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 33 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 200 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 90 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 467 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope AA 1-30, N-Term
(75), (71), (48), (39), (37), (36), (29), (29), (28), (28), (23), (23), (22), (21), (20), (19), (17), (15), (15), (15), (15), (15), (14), (14), (11), (9), (9), (8), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(819), (426), (402), (26), (25), (17), (16), (13), (9), (7), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(783), (41), (14), (7), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(493), (365), (216), (182), (156), (89), (51), (33), (19), (18), (17), (7), (2), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN392261) Images
Specificity This RAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human RAF1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This RAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human RAF1.
Clone RB3329
Isotype Ig

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN392261) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Synonyms: RAF proto-oncogene serine/threonine-protein kinase, cRaf
Molecular Weight 73051 DA
Gene ID 5894
UniProt P04049
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN392261) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN392261) Images back to top
Format Liquid
Concentration 0.44 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-RAF1 Antibody (ABIN392261)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Chong, Guan: "Regulation of Raf through phosphorylation and N terminus-C terminus interaction." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36269-76, 2003

Alavi, Hood, Frausto, Stupack, Cheresh: "Role of Raf in vascular protection from distinct apoptotic stimuli." in: Science (New York, N.Y.), Vol. 301, Issue 5629, pp. 94-6, 2003

Fringer, Grinnell: "Fibroblast quiescence in floating collagen matrices: decrease in serum activation of MEK and Raf but not Ras." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20612-7, 2003

Oehrl, Rubio, Wetzker: "Serine 338 phosphorylation is dispensable for activation of c-Raf1." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17819-26, 2003

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN392261) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 1-30) (ABIN392261) Western blot analysis of anti-RAF1 Pab (ABIN392261) in Jurkat cell lysate. RAF1 (arro...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (AA 1-30) (ABIN392261) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...