anti-Human RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
Middle Region
80
74
47
47
46
43
41
34
34
28
27
23
21
19
18
18
15
15
15
15
15
15
7
7
7
6
6
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
834
411
386
24
23
16
15
13
8
7
7
5
4
3
2
1
1
1
1
Host
Rabbit
779
63
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
512
365
221
172
156
104
52
49
20
18
18
7
2
2
2
1
Options
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Catalog No. ABIN2792055
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906104 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
2.906104 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
2.906104 ABIN222938 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 1
2.906104 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.906104 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.906104 ABIN2705627 IC IF WB Rabbit Center Log in to see Polyclonal
2.906104 ABIN2957891 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
2.906104 ABIN392261 FACS IF IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
2.906104 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
2.906104 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
2.906104 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
2.906104 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
2.906104 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
2.906104 ABIN969560 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
2.906104 ABIN1532404 ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN1532444 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN1532993 ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN1533110 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.906104 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope Middle Region
(80), (74), (47), (47), (46), (43), (41), (34), (34), (28), (27), (23), (21), (19), (18), (18), (15), (15), (15), (15), (15), (15), (7), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(834), (411), (386), (24), (23), (16), (15), (13), (8), (7), (7), (5), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(779), (63), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(512), (365), (221), (172), (156), (104), (52), (49), (20), (18), (18), (7), (2), (2), (2), (1)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN2185168
Sequence SGTQEKNKIR PRGQRDSSYY WEIEASEVML STRIGSGSFG TVYKGKWHGD
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against RAF1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of Human RAF1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Protein Interaction Partner: Prkab1, FYCO1, NPLOC4, TMEM70, COPS7A, DCAF8, PACSIN3, YWHAQ, RANBP9, RB1, PURA, MAP2K2, MAP2K1, MAPK3, MAPK1, PRKAR1A, MYBPC1, HSP90AA1, AKT1, FAM96B, MRAS, HUWE1, SHOC2, UBC, CDC37, IRS4, GPRASP2, STUB1, SH3KBP1, HIPK4, CDK20, YWHAB, TBXA2R, KRAS, YWHAE
Protein Size: 567
Molecular Weight 62 kDa
Gene ID 5894
NCBI Accession XP_005265415
UniProt B4E0X2
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Middle Region) antibody (ABIN2792055) Host: Rabbit Target Name: RAF1 Sample Type: Jurkat Whole Cell lysates Antibody Diluti...