You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
C-Term
74
62
56
39
36
34
27
27
26
26
23
22
21
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
788
337
321
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
741
39
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
460
352
204
181
132
79
40
30
18
15
12
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN222938
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
0.13341247 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
0.13341247 ABIN781705 EIA WB Chicken N-Term Log in to see Polyclonal 2
0.13341247 ABIN392261 FACS IF IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.13341247 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13341247 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13341247 ABIN969560 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
0.13341247 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
0.13341247 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
0.13341247 ABIN126882 WB Mouse IgG1 Log in to see PBB-1
0.13341247 ABIN389747 DB WB Rabbit Ig pSer338 Log in to see Polyclonal
0.13341247 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
0.13341247 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
0.13341247 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
0.13341247 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
0.13341247 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
0.13341247 ABIN2855608 WB Rabbit IgG Center Log in to see Polyclonal
0.13341247 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN498598 IF WB Rabbit Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 85 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 7 Antibodies
Immunofluorescence (IF) 21 Antibodies 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 121 Antibodies 121 Antibodies
Immunohistochemistry (IHC) 76 Antibodies 86 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies 53 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 192 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope C-Term
(74), (62), (56), (39), (36), (34), (27), (27), (26), (26), (23), (22), (21), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(788), (337), (321), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(741), (39), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(460), (352), (204), (181), (132), (79), (40), (30), (18), (15), (12), (2), (2), (2)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images
Blocking Peptide Blocking peptide for this product available: ABIN412894
Specificity The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots.
Cross-Reactivity (Details) It is non cross-reactive with Raf-A and Raf-B.
Purification Affinity purified
Immunogen Synthetic peptide mapping to the C-terminus of human Raf-1
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Antigen
Alternative Name Raf1 (RAF1 Antibody Abstract)
Background Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Synonyms: RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
Gene ID 5894
UniProt P04049
Research Area Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Application Notes Western blot analysis (1-4 μg/mL), Immunoprecipitation (15-25 μg/mL) and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN222938) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN222938)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Ito, Nagayasu, Okumura-Noji, Lu, Nishida, Miura, Asai, Kheirollah, Nakaya, Yokoyama: "Mechanism for FGF-1 to regulate biogenesis of apoE-HDL in astrocytes." in: Journal of lipid research, Vol. 48, Issue 9, pp. 2020-7, 2007

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (C-Term) (ABIN222938) Western blot With Raf-l antibody, using rat kidney lysate (60 µg).