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anti-Mouse (Murine) RAF1 antibody for Immunohistochemistry

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
pSer259
74
61
56
39
36
34
27
26
26
26
23
23
20
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
787
336
318
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
738
39
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
457
351
204
181
132
79
39
30
18
16
11
2
2
2
Supplier
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Catalog No. ABIN1531958
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22915025 ABIN1532077 IHC ELISA Rabbit IgG pSer289 Log in to see Polyclonal 1
0.22915025 ABIN1532076 IHC ELISA Rabbit IgG pThr269 Log in to see Polyclonal 1
0.22915025 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.22915025 ABIN1533109 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
0.22915025 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.22915025 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
0.22915025 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
0.22915025 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
0.22915025 ABIN2469686 IHC WB Rabbit IgG Ser259 Log in to see Polyclonal
0.22915025 ABIN1532444 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN1533110 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
0.22915025 ABIN2500166 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
0.22915025 ABIN2500173 IHC ELISA Rabbit IgG pThr269 Log in to see Polyclonal
0.22915025 ABIN2500183 IHC ELISA WB Rabbit IgG Ser189 Log in to see Polyclonal
0.22915025 ABIN2500184 IHC ELISA WB Rabbit IgG Ser296 Log in to see Polyclonal
0.22915025 ABIN2500185 IHC ELISA WB Rabbit IgG Ser43 Log in to see Polyclonal
0.22915025 ABIN2500186 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
0.22915025 ABIN2500188 IHC ELISA WB Rabbit IgG Ser259 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunohistochemistry

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 85 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 20 Antibodies 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 121 Antibodies 121 Antibodies
Immunohistochemistry (IHC) 76 Antibodies 86 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies 53 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 191 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer259
(74), (61), (56), (39), (36), (34), (27), (26), (26), (26), (23), (23), (20), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(787), (336), (318), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(738), (39), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(457), (351), (204), (181), (132), (79), (39), (30), (18), (16), (11), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1531958) Images
Specificity C-RAF (Phospho-Ser259) Antibody detects endogenous levels of C- RAF only when phosphorylated at Ser259.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser259.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN1531958) Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background Molecular weight of target protein: 73 kDa
Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto- oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Molecular Weight 73 kDa
Gene ID 5895
OMIM 164760
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN1531958) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.159131 (NCBI Gene Symbol: RAF1)

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN1531958) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN1531958)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

King, Sun, Diaz, Barnard, Miao, Bagrodia, Marshall: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1531958) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259) (ABIN1531958) Western blot analysis of extracts from K562 cells, using C-RAF (Phospho-Ser259) Antib...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259) (ABIN1531958) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C-RA...