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anti-Mouse (Murine) RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Reactivity
Human, Mouse (Murine), Rat (Rattus)
779
331
313
18
18
10
5
4
4
2
2
1
1
Host
Rabbit
729
38
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
447
351
199
181
132
74
37
29
18
16
9
2
2
2
Supplier
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Catalog No. ABIN498113
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.33929166 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
0.33929166 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
0.33929166 ABIN499021 IHC (p) WB Rabbit Log in to see Polyclonal
0.33929166 ABIN712736 IF (p) IHC (p) WB Rabbit IgG AA 330-345, pSer339 Log in to see Polyclonal
0.33929166 ABIN498802 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
0.0008997883 ABIN733208 IF (p) IHC (p) WB Rabbit IgG AA 25-60 Log in to see Polyclonal 1
0.0008997883 ABIN197102 IHC (p) WB Rabbit Ser259 Log in to see Polyclonal 6
0.0008997883 ABIN196618 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal 5
0.0008997883 ABIN319325 IHC (p) WB Rabbit Ser338 Log in to see Polyclonal 3
0.0008997883 ABIN271798 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008997883 ABIN271937 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008997883 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
0.0008997883 ABIN302942 IHC (p) WB Rabbit IgG pSer259 Log in to see Polyclonal
0.0008997883 ABIN318109 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
0.0008997883 ABIN127014 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008997883 ABIN127169 IHC (p) WB Rabbit pSer259 Log in to see Polyclonal
0.0008997883 ABIN733628 IF (p) IHC (p) WB Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008997883 ABIN733630 IHC (p) WB Biotin Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008997883 ABIN733637 IHC (p) WB HRP Rabbit IgG Tyr340, pSer338 Log in to see Polyclonal
0.0008997883 ABIN733210 IHC (p) WB Biotin Rabbit IgG AA 25-60 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 440 Antibodies 200 Antibodies 186 Antibodies
RNA Interference (RNAi) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 16 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies 50 Antibodies 46 Antibodies
Immunohistochemistry (IHC) 198 Antibodies 82 Antibodies 72 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies 121 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(779), (331), (313), (18), (18), (10), (5), (4), (4), (2), (2), (1), (1)
Host Rabbit
(729), (38), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(447), (351), (199), (181), (132), (74), (37), (29), (18), (16), (9), (2), (2), (2)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Specificity This antibody detects endogenous levels of C-Raf protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Activation of Raf kinase activity is induced by several events including phosphorylation at several sites and binding of GTP-bound Ras and/or 14-3-3 proteins. The c-Raf antibody is useful as a positive control and for measuring total c-Raf protein levels to compare signals obtained with the c-Raf PSSAs.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight approx. 73 kDa
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN498113) Immunohistochemistry analyzes of C-Raf antibody in paraffin-embedded human bre...
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (ABIN498113) Western blot analysis of C-Raf antibody in extracts from 293 cells.