We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Western Blotting (WB)||454 Antibodies||208 Antibodies||194 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||17 Antibodies||4 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||79 Antibodies||53 Antibodies||49 Antibodies|
|Immunohistochemistry (IHC)||207 Antibodies||87 Antibodies||77 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||132 Antibodies|
|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RAF1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Raf-1 protein. (region surroundign Arg282)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling Images back to top
|Alternative Name||RAF1 (RAF1 Antibody Abstract)|
|Background||Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may even play the role of the messenger from membrane tyrosine kinases to Raf-1.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf|
|Molecular Weight||approx. 73 kDa|
|Research Area||Signaling, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry.1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top