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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||85 Antibodies||86 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||22 Antibodies||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||121 Antibodies||121 Antibodies|
|Immunohistochemistry (IHC)||76 Antibodies||86 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||49 Antibodies||53 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||192 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RAF1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412894|
|Specificity||The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots.|
|Cross-Reactivity (Details)||It is non cross-reactive with Raf-A and Raf-B.|
|Immunogen||Synthetic peptide mapping to the C-terminus of human Raf-1|
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
|Alternative Name||Raf1 (RAF1 Antibody Abstract)|
Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
Synonyms: RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
|Research Area||Cancer, Apoptosis/Necrosis, Signaling, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN222938) Images back to top
Western blot analysis (1-4 μg/mL), Immunoprecipitation (15-25 μg/mL) and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN222938) Images back to top
|Buffer||100 μg (0.2 mg/mL) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-RAF1 Antibody (ABIN222938)Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
|Product cited in:||
Ito, Nagayasu, Okumura-Noji, Lu, Nishida, Miura, Asai, Kheirollah, Nakaya, Yokoyama: "Mechanism for FGF-1 to regulate biogenesis of apoE-HDL in astrocytes." in: Journal of lipid research, Vol. 48, Issue 9, pp. 2020-7, 2007
ImagesProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN222938) back to top