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anti-Mouse (Murine) RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
N-Term
74
62
55
39
37
34
27
27
26
26
23
23
21
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
791
337
321
18
18
10
5
4
4
2
2
2
1
1
Host
Chicken
743
41
16
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
462
355
207
182
132
79
40
32
17
16
12
2
2
2
Supplier
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Catalog No. ABIN781705
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12935057 ABIN222938 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.12935057 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
0.12935057 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
0.12935057 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12935057 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12935057 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
0.12935057 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
0.12935057 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
0.12935057 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
0.12935057 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
0.12935057 ABIN498112 IHC (p) WB Rabbit Log in to see Polyclonal
0.12935057 ABIN499021 IHC (p) WB Rabbit Log in to see Polyclonal
0.12935057 ABIN498598 IF WB Rabbit Log in to see Polyclonal
0.12935057 ABIN498113 IHC (p) WB Rabbit Log in to see Polyclonal
0.12935057 ABIN498893 IHC (p) WB Rabbit Log in to see Polyclonal
0.12935057 ABIN1870562 IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
0.12935057 ABIN1533110 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN1532993 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN1532404 ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 450 Antibodies 206 Antibodies 192 Antibodies
RNA Interference (RNAi) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 16 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies 53 Antibodies 49 Antibodies
Immunohistochemistry (IHC) 206 Antibodies 86 Antibodies 76 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies 121 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope N-Term
(74), (62), (55), (39), (37), (34), (27), (27), (26), (26), (23), (23), (21), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(791), (337), (321), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Chicken
(743), (41), (16), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(462), (355), (207), (182), (132), (79), (40), (32), (17), (16), (12), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN781705) Images
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Affinity chromatography purified via peptide column
Immunogen 19 amino acid peptide near the amino terminus of human C-raf

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN781705) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Gene ID 5894
NCBI Accession NP_002871
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN781705) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN781705) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-RAF1 Antibody (ABIN781705)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Chang, Steelman, Shelton, Lee, Navolanic, Blalock, Franklin, McCubrey: "Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway (Review)." in: International journal of oncology, Vol. 22, Issue 3, pp. 469-80, 2003

Howe, Leevers, Gómez, Nakielny, Cohen, Marshall: "Activation of the MAP kinase pathway by the protein kinase raf." in: Cell, Vol. 71, Issue 2, pp. 335-42, 1992

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN781705) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (N-Term) (ABIN781705) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (N-Term) antibody