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anti-Mouse (Murine) RAF1 antibody for Western Blotting

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pSer43
72
69
47
39
38
37
28
28
28
27
22
22
20
20
19
18
16
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
790
409
384
25
23
16
16
13
8
8
8
5
4
2
2
2
2
1
1
1
Host
Rabbit
756
41
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
473
342
202
172
156
95
49
33
19
18
17
7
2
2
2
1
Options
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Catalog No. ABIN1531571
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1986368 ABIN222938 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal 1
3.1986368 ABIN781705 EIA WB Chicken N-Term Log in to see Polyclonal 2
3.1986368 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
3.1986368 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.1986368 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.1986368 ABIN658201 FACS IHC (p) WB Rabbit Ig AA 86-113, N-Term Log in to see Polyclonal
3.1986368 ABIN2792055 WB Rabbit Middle Region Log in to see Polyclonal
3.1986368 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
3.1986368 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
3.1986368 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
3.1986368 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
3.1986368 ABIN3182575 ELISA IHC WB Rabbit IgG pSer296 Log in to see Polyclonal
3.1986368 ABIN3186668 ELISA WB Rabbit IgG Thr560 Log in to see Polyclonal
3.1986368 ABIN3182138 ELISA IHC WB Rabbit IgG pSer259 Log in to see Polyclonal
3.1986368 ABIN3182330 ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal
3.1986368 ABIN3182404 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
3.1986368 ABIN3186669 ELISA IF WB Rabbit IgG Thr566 Log in to see Polyclonal
3.1986368 ABIN3186674 ELISA IHC WB Rabbit IgG Thr596 Log in to see Polyclonal
3.1986368 ABIN3186667 ELISA IF IHC WB Rabbit IgG Thr56 Log in to see Polyclonal
3.1986368 ABIN3186671 ELISA WB Rabbit IgG Thr573 Log in to see Polyclonal

Top referenced anti-RAF1 antibody for Western Blotting

Similar anti-RAF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 6 Antibodies 6 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Western Blotting (WB) 448 Antibodies 255 Antibodies 233 Antibodies
RNA Interference (RNAi) 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 17 Antibodies 10 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 95 Antibodies 81 Antibodies 71 Antibodies
Immunohistochemistry (IHC) 186 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer43
(72), (69), (47), (39), (38), (37), (28), (28), (28), (27), (22), (22), (20), (20), (19), (18), (16), (15), (15), (15), (15), (15), (14), (14), (10), (8), (8), (7), (6), (6), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(790), (409), (384), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(756), (41), (12), (6), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(44), (44), (43), (29), (29), (29), (13), (13), (12), (12), (12), (12), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(473), (342), (202), (172), (156), (95), (49), (33), (19), (18), (17), (7), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1531571) Images
Specificity C-RAF (Phospho-Ser43) Antibody detects endogenous levels of C-RAF only when phosphorylated at Ser43.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser43.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN1531571) Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background Molecular weight of target protein: 73 kDa
Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto- oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Molecular Weight 73 kDa
Gene ID 5894
OMIM 164760
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN1531571) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.159130 (NCBI Gene Symbol: RAF1)

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN1531571) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-RAF1 Antibody (ABIN1531571)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN1531571) back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer43) (ABIN1531571) Western blot analysis of extracts from NIH-3T3 cells, using C-RAF (Phospho-Ser43) Ant...