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anti-Rat (Rattus) RAF1 antibody for Immunofluorescence

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
pSer621
74
62
56
39
36
34
27
27
26
26
23
23
21
20
19
17
16
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
788
337
321
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
741
39
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
460
351
204
181
132
79
39
30
18
16
12
2
2
2
Supplier
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Catalog No. ABIN1531386
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.32040194 ABIN2705627 IC IF WB Rabbit Center Log in to see Polyclonal
0.32040194 ABIN2500192 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
0.32040194 ABIN2500189 IF IHC ELISA WB Rabbit IgG Ser621 Log in to see Polyclonal
0.32040194 ABIN2403452 IF WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN271937 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN498598 IF WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN447019 ICC IF WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN2957891 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.0010840179 ABIN3186667 ELISA IF IHC WB Rabbit IgG Thr56 Log in to see Polyclonal
0.0010840179 ABIN3186669 ELISA IF WB Rabbit IgG Thr566 Log in to see Polyclonal
0.0010840179 ABIN3182769 ELISA IF IHC WB Rabbit IgG pSer621 Log in to see Polyclonal
0.0010840179 ABIN1844887 ICC IF IHC WB Rabbit pSer621 Log in to see Polyclonal
0.0010840179 ABIN1844889 ICC IF IHC WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN1575147 IF IHC ELISA WB Rabbit pSer621 Log in to see Polyclonal
0.0010840179 ABIN1575153 IF IHC ELISA WB Rabbit Ser621 Log in to see Polyclonal
0.0010840179 ABIN2988466 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN1847970 ICC IF IHC ELISA WB Rabbit C-Term, Ser621 Log in to see Polyclonal
0.0010840179 ABIN2857837 ICC IF IHC WB Rabbit pSer621 Log in to see Polyclonal
0.0010840179 ABIN2858758 ICC IF IHC WB Rabbit Log in to see Polyclonal

Similar anti-RAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 85 Antibodies 86 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 7 Antibodies
Immunofluorescence (IF) 21 Antibodies 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 121 Antibodies 121 Antibodies
Immunohistochemistry (IHC) 76 Antibodies 86 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies 53 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 192 Antibodies
Flow Cytometry (FACS) 4 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer621
(74), (62), (56), (39), (36), (34), (27), (27), (26), (26), (23), (23), (21), (20), (19), (17), (16), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(788), (337), (321), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(741), (39), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(460), (351), (204), (181), (132), (79), (39), (30), (18), (16), (12), (2), (2), (2)
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling Images
Specificity C-RAF (Phospho-Ser621) Antibody detects endogenous levels of C- RAF only when phosphorylated at Ser621.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser621.
Isotype IgG

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-RAF (RAF1 Antibody Abstract)
Background Molecular weight of target protein: 73 kDa
Synonyms: C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto- oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Molecular Weight 73 kDa
Gene ID 5894
OMIM 164760
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.159130 (NCBI Gene Symbol: RAF1)

Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer621) (ABIN1531386) Western blot analysis of extracts from HeLa cells treated with UV 5', using C-RAF (Ph...
Immunohistochemistry (IHC) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer621) (ABIN1531386) Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF ...
Immunofluorescence (IF) image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer621) (ABIN1531386) Immunofluorescence analysis of HeLa cells, using C-RAF (Phospho-Ser621) Antibody. The...